DNA sequence >NC_022785, from 3819652 to 3819801 (150 bp); Streptomyces rapamycinicus NRRL 5491 CCCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNN Translate to protein Restriction digest BLAST Design primers with primer3