Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 3725856
End position: 3729656
Length: 3801

DNA sequence
>NC_022785, from 3725855 to 3729655 (3801 bp); Streptomyces rapamycinicus NRRL 5491 GTGGCGGCCGTCCTGCTGGCGGTGACGGCCCAGGGCGGTGCGGTCGCCGCCCCCGCCGGCCCGGACTCCG CGCACCACGCCGACCGCACCTTCGCCTCGTCCTTCGAGTCGGGCGATCCCCAGCCCGACTGGCGGAACAC GGTCGAGACCGGCCCCGACGGCAAGAAGAAGGCGTCGGGAGTCGACGGCGGCTACTCCTCCGGGATCCCC GGCAATGTCACCGACAAGGTCACCGAGGTCCGCGCGAGCGACGAGAACACCGACGGCGGGGAGATCAAGG AGAACCTCGTCGACGGCGAGGTCACCAGCAAGTGGCTGGCCTTCGACACCACCGCCTGGCTCGAGTTCGA CCTGAGCGAGCCGGTGAAGGCCGTCCGCTACGCCCTCACCTCCGCCAACGACGCCCCCGGCCGGGACCCG AAGGACTGGACCCTCAAGGGCTCGGCCAACGGCTCCGACTGGACGACGCTCGACACCCGTAAGGACGAGA CGTTCTCCTCGCGCCAGCAGACGCGAGAGTTCTCCTTCGACAGTTCCACGGCTTATCAGCACTTCCGGCT GGAGATCACCCGCAACGCCGGGGACGACATCGTCCAGCTCGCGGAGGTGCAGTTCGCGAACGGCGACACC ACTCCGCCCGCGGCCTCCGACATGCAGTCCCAGATCGACCGCGGCCCGACCGGCTCCCCCACCGCCAAGG CCAACGCGGGCTTCACCGGCACCCGCGCCCTGCGCTACGCGGGCACCCACAAGCCGGATGGCCGGGCATA CTCGTACAACAAGGTCTTCGACGTCGACACGGCCGTCACCCGGGACACCCAGCTGTCCTACCGGATCTTC CCGTCGATGACCGAGACGGACCTGAACTACCCGGCCACCCATGTCTCCGTCGACCTGGCCTTCACCGACG GCACCTACCTCAGCGAACTGCCCGGCGCGGTCGACCAGCACGGCGCGCGGATGAGCCCGCAGGGCCAGGC CGACTCCAAGACGCTCTACGTCAACCAGTGGAACCACAAGGAGTCGCGGATCGGCGCGGTCGCCGCGGGC AAGACGGTCGACCGGATCCTCGTGGCCTACGACTCCCCCAAGGGCCCCGCGCAGTTCCGCGGCTGGGTGG ACGACATCGCGATCGCCCCCAAGGCGCCCGAGAAGCCGCTGGCGCACCTGTCGGACTACGCGCTGACCAC CCGCGGCACCAACTCCAGCGGCAGCTTCTCGCGCGGCAACAACTTCCCGGCCACCGCCGTCCCCAATGGC TTCAACTTCTGGACGCCGGTGACCAACGCGGGCTCCACCGACTGGCTCTACCAGTACGCCCGCGCCAACA ACGCCGACAACCTCCCCACCATCCAGTCGTTCAGCGCCAGCCATGAGCCCAGCCCCTGGATGGGCGACCG GCAGACCTTCCAGGTCATGCCGTCCGGGGCGAGCGACACCCCCGACGCCGGGCGCACCGCCCGCGCGCTC CCCTTCCGCCATGAGAAGGAGACCGCCCGCCCCTACTACTACGGGGTGACCTTCGAGAACGGCCTCACCG CCGAGATGGCGCCCACCGATCACGCGGCGGCCATGCGCTTCACCTATCCGGGCGAGGACGCGAGCGTCAT CTTCGACAACGTCAACAACAACGGCGGGCTGACCCTCGACGCCGAGCACGGCGTCGTCACCGGCTACTCC GACGTCAAGAGCGGACTGTCCACCGGCGCCAGCCGGCTGTTCGTCTACGGCGTCTTCGACACCCCGGTGA GCGCGAGCGGCAAGCTGGAAGGAGGCGGCGGCAAGGATGTCACCGGCTACTTCCGCTTCAAGCCGGGGAA GGACCGCACGGTCACCCTCCGGCTCGCCACCTCGCTGATCGGCGTCGACCAGGCGAAGGCCAACCTCGAC CGGGAGCTGCCCGCGTCGCGGTCGTTCGGCCAGGTCAAGCGGGCCGCCCAGGCGGAGTGGGACAAGATCC TGGGCCGGGTCGAGGTCGAGGGCGCGGACCGCGGCCAGCTCACCTCGCTCTACTCCAGCCTCTACCGGCT GTACCTCTATCCCAATTCCGCTTTCGAGAACACCGGGACGGCGGCGAAGCCCCGCGACCAGTACGCGAGC GCCTTTTCGCCGGCCGCCGGGGAGAACACCCCGACCCATACCGGCGCGAAGATCGTCGACGGCAAGGTGT ACGTCAACAACGGCTTCTGGGACACCTACCGCACCACCTGGCCCGCCTACTCCCTGCTCACCCCGAAGCG GGCGGGCACGATGGTCGACGGCTTCGTGCAGCAGTACAAGGACGGCGGCTGGATATCCCGCTGGTCCTCA CCGGGCTACGCCGATCTGATGACCGGCACCAGCTCGGACGTGGCCTTCGCCGACGCGTATCTGAAGGGTG TGCGGTTCGACGCCGAGGCCGCGTACGAGGCCGCGCTGAAGAACGCCACCGCCGTGCCACCCACGTCGGG CGTCGGCCGGAAGGGCATGGCCAGCTCGCCCTTCCTCGGCTACACCAGCACCGAGACCCATGAGGGGCTG TCCTGGGCGCTGGAGGGCTATCTCAACGACTTCGGCCTGGCCAACATGGGCAAGGCGCTCTACGCGAAGA CGAAGAAGATCCGCTACAAGGAGGAGTCCGAGTACTTCCTGCACCGTGCGCAGGGCTATGTCTCGCTCTT CGACGACAAGGCGGGCTTCTTCCAGGGCAAGGACGCCAATGGAAAGTGGCGCGTGCCCTCCGACAAGTAC GACCCCCGGATCTGGGGCTACGACTACACCGAGACCAACGGCTGGGGCTACGCCTTCACCGCGCCCCAGG ACAGCCGGGGCCTGGCCAACCTCTACGGCGGCCGCGCCGGTCTGGCGAAGAAGCTGGACACCTACTTCTC CACCCCGGAGACCGCCTCGCCGGAGTTCGCCGGTTCCTACGGCGGCGTCATCCACGAGATGACCGAGGCG CGGGACGTCCGCATGGGCATGTACGGCCACAGCAACCAGGTCGCCCACCACGTGGCGTACATGTACGACG CGGCGGGCCAGCCGTGGAAGACCCAGGAGAAGGTGCGCGAGGTGCTCTCCCGCCTCTACACCGGCAGCGA GATCGGCCAGGGCTACCACGGCGACGAGGACAACGGCGAGCAGTCCGCCTGGTACCTCTTCAGCTCGCTG GGCTTCTATCCGCTGGTGATGGGCGGCTCGGAGTACGCGGTCGGATCGCCGCAGTTCACCAAGGCCACGG TGCATCTGGAGAACGGCCGCGACCTCGTCGTCAAGGCCCCGCGCAACAGCGCGCGGAACGTGTACGTCCA GAGTCTGAAGGTCAACGGCAAGGCGTGGAACTCCACCGCGCTGCCGCATGACCTGATCGCCCGCGGCGGC ACGCTGGAGTTCGCCATGGGCGCCAAGCCGTCCTCCTGGGGCACCGGCGCGAACGCCGCGCCGTCCTCCA TCACCAAGGACGACAAGGTGCCCACCCCGCGCCACGACCTGACGGTGCCGAGCGGCGGTACGGCGCTGCT GGACAACACCTCGGGCACCGAGGCGGCCTTCGAGTCGGTGGACCTGCCGGTCACGAAGCCGGGCCGCGCG GTGCAGTACACCCTGACCTCGTCGGACCGCGGCAAGGCCCCGACGGGATGGGTGCTCCAGGGCTCTCCGG ACGGCGAGAAGTGGCGGGACCTGGACCGCAGGTCGGGCGAGTCCTTCAGCTGGGACAAACAGACCCGCGT CTTCTCGGTCAAGAACCCCGGCGTCTATGAGCACTACCGCCTGGTGCCGGACGGCAAGGCGACCCTGGCG GAGGTGGAACTGCTGAGCTGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 3725856-3729656	Sequence	M271_14400 - alpha-1 2-mannosidase