DNA sequence >NC_022785, from 3530483 to 3530642 (160 bp); Streptomyces rapamycinicus NRRL 5491 CGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNN Translate to protein Restriction digest BLAST Design primers with primer3