DNA sequence >NC_022785, from 3530480 to 3530654 (175 bp); Streptomyces rapamycinicus NRRL 5491 CCGCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN Translate to protein Restriction digest BLAST Design primers with primer3