Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 322599
End position: 324455
Length: 1857

DNA sequence
>NC_022785, from 322598 to 324454 (1857 bp); Streptomyces rapamycinicus NRRL 5491 TCACGCGGTGTCCTCGGTCAGCCGGGGCCAGGCGGCCTCGCGCAGCAGGCGCAGGCCGTTGAGGCCGACG ATGACCGTGGAGCCCTCATGTCCGGCGACGCCCAGGGGCAGAGGGAGGGTGCCGATCAGGTCCCAGGCCA CCAGGACGGTGATGAACGCCCCGGCGATGGCCAGGTTCTGGACGACCAGGCGGCGCGCGGTGCGTGAGAG CCGCACGACGGACGGGACAGTGGCGAGTTCGTCTCGGACCACGACAGCGTCGGCGGTCTCCAGAGCAAGG TCGGAGCCGGCCTTGCCCATGGCGATCCCCACGTGTGCGGCGGCTATCGCAGGCGCGTCGTTGACGCCGT CGCCGACGACCAGCGCCCGTCGCCCCTGGGTCTCCCACTCCTTGACGGCGGCCAGCTTGTCCTGGGGCAG CAGCCCGGCGCGGATCTCAGTGATGCCGACCTCGGCGGCCAGACGCCGGGCCGCACGCTCGTTGTCGCCG GTCAGCAGCACCGGGGCGCGGCCGGTCAACCGGGTGAGGGCAGCGACCGTGGCCGCCGCGTCCGGCCGCA GCCGGTCGGCGATGCCCAGCACCCCTACCGGCGTGCCGTCGCGGAATACGGCGACCGCGGTGCGCCCGGC CTCCTCCAGGCGGGCCACGACCTTCCGAGGTGGCTCGTGGGCGGCGCCGTTCAGCAGGCGGGCGGGCGAG CCGACCGTGACGGTGTGTCCGTCGACCTCGGCGGTGACGCCCTGCCCGGGGCTGGAGGTGAAGTCGTCCG CATCGGCCAGGGGCAGGCCGCGTTCGCCGGCGGCGGTGACCACGGCGCGGGCGAGCGGGTGCTCACTCGG GTGCTCGGTGGAAGCAGCCAGCCGCAGAAGGGCGCTCTCGTCCACGTCGCTGCCGGGAAGCGGGCACAGG TCGGTGACGTAGGGGGTGCCTTCGGTGAGGGTGCCGGTCTTGTCCAGGGCGACGATGTCGATCTGCCCGA GGCGCTCCATGATGACGGAGGACTTGGCGAGCACCCCGCGGCGGCCGGCGTTGGCGATGGCGGACAGCAG CGGCGGCATGGTGGAGAGCACCACCGCGCACGGCGAGGCCACGATCATGAACGTCATCGCCCGCAGCAGC GCGGAGGAGAGCGTGTCGCCGAAGGCGAAGGGGACGGCGAAGACGGCGAGGGTGGCGATGACCATGCCGA TCGAGTAGCGCTGCTCGATCTTCTCGATGAGCAGCTGGGTGGGAGCCTTGGTCGCAGAGGCTTCCTCAAC CATCTTCACGATCCGGGCGATCACCGAATCCGACGGGTCCCGCTCGACCCGTACGCGCAGCGCGCCGGTG CCGTTGACGGTGCCGGCGAACACCTCGTCCCCCGCCTGCTTGGCCACGGGCAGGGGCTCGCCGGTGATGG TGGCCTGGTCGACGTCGCTCGTCCCGTCGAGGACCTGGCCGTCGGCGCAGATGCGCTCCCCGGGCCGGAT CAGGATGGTGTCCCCGACCGCGAGGTGCTTGGTGGCGACGTTCTCCTCGGTGCCATCCGGCAGGAGGCGG GTGGCGGTGGTGGGGGCGACGTCGAGCAGGCCGCGCACGGAGTCGGTGGTGCGGGCGGTGGCGACGGCTT CCAGGGCACCGGAGGTGGCGAAGATGACGATCAGCAGCGCCCCGTCGAGGAGCTGCCCGATCGCGGCGGC TCCGAGGGCGGCGGCGACCATCAGCAGGTCAACGTCCAGCGTCTTGTCCTTCAGCGCCTTCAGGCCCTCC CAGCCCGGCTCCCAGCCACCGGTCAGGTAGGTCGCGGCGAACAGCGTCCCCCACAGCCACGCCGGACCGT CCAGCAGGTAGACGGGCAGAGCGAGCAGGAACAGCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 322599-324455	Sequence	M271_01305 - cation-transporting ATPase (Protein is coded in complementary strand; from 324455 to 322599)