Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 3225941
End position: 3228811
Length: 2871

DNA sequence
>NC_022785, from 3225940 to 3228810 (2871 bp); Streptomyces rapamycinicus NRRL 5491 TCACTCACGCTCGGAGGGACGGTCGTCGGAGGGTCGGAGGTGCTGGGCCAGCAACTGGGCGAGATGGGTG CCGCGGACCCCGGCGAGCTGGTCGAGTTGAGTCCGGCAGGAGAACCCGTCGGCCAGCACGACCGCGTCCG CCTCCGCCTTCCGCACGGCCGGCAGCAAAGCCGTCTCCGCCACGGCGACGGAGATCTCGTAGTGTCCCCG CTCGACGCCGAAGTTCCCGGCCAGCCCGCAACAGCCGCCGACGGTGCGTACGGTGGCGCCGGCGTGTCCG AGCAGTTCGGCGTCGGCGGTCCAGCCCATGACGGCGTGCTGGTGGCAGTGCGGCTGTGCGATGCCGCACA CACCGTCGAGCCTCGGCGGTGTCCACGTGGCGGTGGCCGTCAGCAGTTCGGCCAGCGTGCGCGTCGCGGC TCGTACCGCTGTCGCACGCGGGTCGCCGGGCAGCAGTTCGGCCGCGTCCTTGCGCAGTACCGCGGTGCAG GACGGCTCCAGCCCGACGACGGGGATCCCTTCCTCCAGGGCGGGTTCGAGCGCGTCCAGGCTGCGCCGCA GTTGGGCGCGGGCTCCATCGAGCTGGCCGGTGGAGATCCAGGTGAGGCCGCAGCAGACGGGCCCGTCGGT GAGCCGCACGGCGTACCCGGCGTCCTCGAGCACCGCCACGGCCGCCTCCCCGACCTCCGGGCTGAAGTGG TCGGTGAACGTGTCGAACCACAGGAGCACCGGTGTCCCGGTGGCGGCGGATCGCCCGGCGAACCACTGCC GGAAGGTGGGGGTGGCGAACGCGGGCAGCTCCCGGCGGGCGTCGACACCTCCCAGCCGCTTGACGAGCGC CGCGGGCCACGGCCTGCTCAGCACGGCGTTGGCCACGCGGGGCAGGCGTGAGGCCAGGCGTGCCCAGCGG GGCAGCCATCCCAGGGCGTAGTGGGCGGCCGGTCTGATCCGGCGGCGGTAGCGCTGGTGGAGCGCCTCGG CCTTGTAGGTGGCCATGTCCACACCGGCCGGGCAGTCGGCCGAGCAGCCCTTGCAGGACAGGCACAGGTC CAGTGCCTGCGCCACGTCATCCGAGCGGAATCCTGTGATCAGCGAGCCGTTCGCGACCTCTTGGAGTACC CGGGCCCGGCCCCGGGTGGAGTCCTTCTCGTCCCGTGTGGCCAGATAGGACGGGCACATGACACCGCCCG CGTCCGTGGTGTCCGATCGGCACTTGCCGATCCCGACGCAGCGGTGCACCGCGTTCGCCAGATCACCTCG GTCGTGCGGGTAGGCGAAGCCGAGGCCGCCGGTCAGTGACCGGGCGGCGGGCGCCCGAAGGTCCGCGTCG AGCGGCGCCGGGTCGGTGAGGACACCGGGGTTGAGCAGGTTGTCCGGGTCGAATACGCGCTTGACCTGGC GGAACGCCTCGATGGCCGCGGCGGAGTACATATACGGCAGCAATTCGCCGCGCGCCCGGCCGTCGCCGTG CTCGCCGGACAGGGAGCCACCGTAGTGGCCGACCAGCCGGGCGGCGTCGACCACGAACTCGCGGTAGCGG TCCGGCGCCGTGGCGAGGGGGAAGTCGATCCTGGCGTGCACACATCCCTCGCCCAGGTGGCCGTACATCA GCCCGTCGACGCCGTGCTCCCGCATCAGCCGGCCGAACGCCCGCAGGTAGTCGCCGAGGCGGACCGGGGG CACCGCGGCGTCCTCCCAGCCGGGCCACGCGGGCTCACCGGCCGGGCTGCGGCCACCGAGCCCGACGCCG TCCTCACGGATCGCCCACAGCGCCCGCGCGGGCCCGCCCGTGACGACCGCGGAGTCGAGGCAGTCGGCGT CCGTGATGAGCTTCGCCGCGTTCGCCCGTGCCTCGGCTTCGGTGTCGCCCGGCGTTTCCACGAAGAGCCA GCCATCTCCCCGGGGGAGATCGGGCACCGCGCCGCCGCCGCGCCGGGAGCGGACGACCTCCACCAGGCGG GCGTCCATTCCCTCGATCGCCACGGGGTGGTGCGGCAGCAGAGCCGGTACCGCATCGGCGGCCGTGGGCA TGTCGGGATAGCCCAGCACCGCCAGTGCCACGGCGGGGGGAGGTTCGACCAGCCTCACCACCGCGCCCAG CACCACACCGAGCGTGCCCTCGCTGCCGACGAGGAACTTCGCCAGATCCGCGCCGTTCTCCGGCAGCAGG TGCTCCAGCGAATAGCCCGACACCTGCCGACCGAACCGCCCGAACTCGGTGCGGATCACCGCCAGGTGCT CGCTGACGAGGTCTTCCAGCGCACGGCCCTTGTCACCCGCGGCGCGCAGGCCGGACCGGCCGAGGCGGCG GGCGTCCAGCCGCTCGCCGTCGGCGGTCAGGGCGGTGAGGGACACGACGTTGTCGGCGGTGCGGCCGTAC CGGAGCGCCCGCGCGCCGCAGGCGTTGTTGCCCAGCGAACCGCCGATCGTGGCGCGGGCATGGGTCGAGG GATCGGGCCCGTACCGCAATCCGTACGGGGCCGCGGCGGCGCTGATGTGATCGAGGATCGCTCCCGGCTG GACGGTGGCCGTCCGCGCCTCGGCGTCCACCAAGAGCACCCGGTTCAGATGCCGGGAGAAGTCCAGGACG ATACCGGTGCCCACCGCGTTGCCCGCGGTCGAGGTCCCGCCGCCCCGGCTGGTGACCGGAACCCCCGCGG AGCGCGCGACCGCGACGGCGGCCGCGACCTCGTCGGGGTGCCGGGGGAAGGCCACGACCGCGGGTACGAC ACGGTAGTTGGAGGCGTCCGACGCGTACTCCGCCCTGCGGCGGGCGCTGACATCGGCTTCTGCCAGTCCG GCACGGCGCAACGCGTGTGCCAGTTCCCGGCGCTCCGCCGGGCCGCCGTCCGGCGCTTTCCGTCCGCTCA T

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 3225941-3228811	Sequence	M271_12340 - hypothetical protein (Protein is coded in complementary strand; from 3228811 to 3225941)
ORF... 3228808-3229782	Sequence	M271_12345 - hypothetical protein (Protein is coded in complementary strand; from 3229782 to 3228808)