Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 316328
End position: 316864
Length: 537

DNA sequence
>NC_022785, from 316327 to 316863 (537 bp); Streptomyces rapamycinicus NRRL 5491 TCAGATGGGAAGGGTCGTTCCAGCCTCGACCTGTTGCCCCAACGCGGACCTGGCGTCGCGGAACAGGGTC CGCCCGTAGTCACGGTACGGAGGCGACTCCGCGTATCGGAAATGATCACGCACCGCGACCCGCGCGGCCT CGGGGTCCCCGGACTCCAACGCCTCGACGATCGCGTCATGCGAGCCGATGCCCCACTGTTCTCCGCTCTG GGGGTCCGGCGGGATGGAGCTGACGATGATCAGCTCCTGGAGCGGTTCGATCAGCAGTTCCAGTGCGGGC AGTCGAAGCACCCTGATGATCGACAGGTGGAGTTGGAGGTGCTCCTGATGCGCCGTCGGCCAGTCCTCGG CACGGACAGCGTCCTCGAACCGCCGCAAGTGCCCGCGCATCGGCCGCAATGCTCCGGGATCGCGTACTCG CGCGGCCTCGGCCGCGAGCGTCGACTCCAGCGCGACCCGGGCGGCCAGAACCTCACCGATGGTGATCTNN NNNNNNNNNNNNNNNNNNNNNNNTGACCCGGGCGGCCAGAACCTCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 316328-316864	Sequence	M271_01270 - hypothetical protein (Protein is coded in complementary strand; from 316864 to 316328)
ORF... 316800-317135	Sequence	M271_01275 - hypothetical protein (Protein is coded in complementary strand; from 317135 to 316800)