Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 3092429
End position: 3095536
Length: 3108

DNA sequence
>NC_022785, from 3092428 to 3095535 (3108 bp); Streptomyces rapamycinicus NRRL 5491 TCACCACCGTGCGGTCACCTTCCCCCGCGGCGCNNNNNNNNNNNNNNNNNNNNNNNNNCGCGAGGGCGGG TCCGCGCGCTGTGCCAGGCGTTCGGGCCGCTCGCTGGGCAGGGTGGTGTCGAGGCCCAGGGCCAGCGCCT CGGCCAGGTGGAGGGCCCCGCGGCCGGTGTCGCCCTGCTCGATCTGGGTGCGGCAGCTGAAGCCGTCCGC GAGCAGCAGGCTGCCGGGGGCGGCCGCGCGCACGGCGGGCAGCACGCCCTGTTCCGCGACGGCCATCGAG ACCTCGTGGTGGCCGCGCTCGAAGCCGAAGTTCCCGGCCAGACCGCAGCAGCCCTCGTCCAGGACGTCGG TGACGAGGTGGGCTCGGCGCATCAGTTCGCGGTCCGGGCCGTCCTTCATGATGGCGTGCTGGTGGCAGTG GGTCTGGACGACGGCCTGCCGGCTGAGCGCGGGTGGCCGCCATCCGTCGGGGGCGTGGTGGACGAGGTGT TCGGCGAAGGTGCGGAACTGCCCGGCCAGCCGCTCCACGTCCTGGTCGGCGGGCATCAGTTCGGGTGCGT CGGCCCGGAACACGGCGGTGCAGGAGGGTTCGAGGCCGATGACCGGAGTGCCCGCCTCGAGGTAGGGCCG CAGCATCCGCAGCGTACGCCGCAACACCTTCTTCGCCGTGGCCAGTTGCCCGGTGGAGATCCAGGTCAGA CCGCAGCACACGGGCTCGGTCGGTACGGCGACCCGGAAGCCGGCGTCCTCCAGCACCCGGACGGCCGAGA TGGCGACCGAGGGGTGGAAGTAGGTGCTGAAGGTGTCGGGCCACACCACCACGGTCCGCGGGTCGGCCGG GTCCGGCTCCTCGGCGCCGCGGGCGCGCCACCACTGGAGGAAGGACCGCGGTGCGAACACCGGTGCCTGG CGCGCGCCGTCCACCCCGGCCAGCCGCTTGCCGAGACCGGCCACGCCCGGCGCCCGGGCCACGGTGTTGA CCAGTGAGGGCGCGATACGGGACAGCCGGGCCCACACCGGCAGCCAGCCCATGGAGTAGTGCGCCGCCGG ACGCGGGCGGCCCGCGTAGTGGTGGGCGAGGAACTCGGCCTTGAGCGTCGCCATGTCGACACCGGTCGGG CAGTCGGACTTGCAGCCCTTGCACGCCAGGCACAGGTCCAGGGCGTCACGGACCTCGGTCGAGCGCCAGC CGTCGGTGACGGCGGAGTCCGGGTGGCCGTCGAGCATCTCGAACAGCAGCCGGGCCCGGCCGCGGGTGGA GTGCTCCTCCTCGTTGGTGGCCCGGTACGACGGGCACATCACGGCGCCGTGGTGGCCGCGGCAGTTGCCG ATGCCGACACAGCGCATGACCGCGCGGGTGAAGGAGTGCTCGTCGTCGGGGTAGCCGAAGTGTGTGTCGG GAGCGGCCGGCCGCCACCCCGGGCCCAGGCGCAGCTGACCGTCGACCGGATTCGGGCCGGTGGCCGTGCC CGTTCCGGTGGCCTTGCCGGGGCCGGTGGCCTTGCTGGGGCCGGTGGCCTTGCCGGGGCTCGGGCCGGTG GCCTTCCCAGGATTCGGGCCGGTGACCTTGCCGGGGTTCATCCGGTTCTCCGGGTCGAACACCGCCTTCA CCTCGCCGAACGCGGTCACCAGGCGCTCGCCGAACATGCGGGTGAGCAGTTCGCCCCGCGCCTGCCCGTC CCCGTGCTCGCCGGACAGCGAGCCGCCGTACGAGACCACCAGGTCTGCGGCGCGTTCCAGGAAGCGGCGG AAGTCGGCCACGCCGTCCGCGGTCTTCATCCCGAACGGGATGCGGGTGTGCACACAGCCCTGCCCGAAGT GGCCGTACAGCGACGGATGGTCGTAGCCGAACTCCGCGAACAGGTCTTTGAGATCGCGCAGATAGTCCCC CAGGCTCTCGGGCGGGACGGCGGAGTCCTCCCAGCCCTCCCAGGTCTCCCGGTCGTCCGGCGGGCGCGCG GTCACCCCGAGGCCCGCCTCACGGGCCCTGAGCATCCGCTGCTCGCGCCGGGGGTCGTCGGAGAAGGCCA CCGTGGCGTCCTTCTCGTCCCGCCCCACCGAGCGCAGCAGCGCGTGCGCCTGCTCGTCGACGCCCTCCTG GGTGTCCCCGCTGAACTGCACCATCAGCCAACTGCCTCCCTCGGGGAGGGAGTTGAGGGATTCGAGATAC GCGTGCTCCTCGCGCATCAGCTGGGCCATCCGCCCGTCGAGCGCCTCCAGTTCGCCGGGTGTGCAGTGCT CCAGGAGCCGGGGCACATCGTCGGCGGCGGTACAGATGTCGTCGTAGCCGAGCACCAGCAGCGACTGGTA CGGGGGCACCGGCACCAGATCCACTTCGGCGCGGATCACCGTCACCAGCGTGCCCTCGCTGCCCACCAGG GCCCGGGCCACGTCGAAGCCGTTCTCCGGCAGCAGCGAGTCCAGGTTGTAGCCGGAGACCCGGCGCGGGA TCTTCGGGTAGCCGCGCCGGATGTCCGCGAGGTACTCGCCGACGACGCGGTCCAGCCCGTCGTAGATCTC GGCCCGGCGGCCACCCTCCGCCGCGATCCGCGCCCGCTCGGCCTTGGACGTCGGCCCCACCCACATGCGC ACGCCGTCGTAGGTGAGGACCTCCAGGCGGCGCACGTTGTCCACGGTCTTGCCGTACGCCTGCGCCGAGG CGCCGCACGAGTTGTTGCCGATCATGCCGCCCAGGGCGCAGTGGCTGTGCGTGGACGGCTTCGGCCCGAA CTGCAGCTTGTAGCCGTCCAGTTGGCGGTTGAGCTCGTCCAGCACCAGCCCCGGCTCCACCACACAGGTG CGCGCCTCGGGATCCACCGAGACCAGGGCGTGGCAGTACTTGCTCCAGTCGATCACCACGGCGGTGTTGG TGCACTGACCGGCCAGGCTGGTGCCGCCGCCCCGGGACGACACGGGCGCGCCGAAGCGGGCGCACACCTC CACCGCCCGCGCCCCGGCCGTCACACTGCGCGGGACGACGACGCCGATGGGCACCTGACGGTAGTTGGAG CCGTCCGTGGCGTACGCGCCCCGGCTGCCCGCGTCAAACCTGACCTCGGCGTCCACCTCCGCACGCAGTG CCCCCTCCAGTCCGCTCGCGGTACGCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 3091299-3092435	Sequence	M271_11790 - hypothetical protein (Protein is coded in complementary strand; from 3092435 to 3091299)
ORF.. 3092429-3095536	Sequence	M271_11795 - dimethylmenaquinone methyltransferase (Protein is coded in complementary strand; from 3095536 to 3092429)