Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 2760285
End position: 2762957
Length: 2673

DNA sequence
>NC_022785, from 2760284 to 2762956 (2673 bp); Streptomyces rapamycinicus NRRL 5491 CTACCGGTCCGATTCCGCGACGAGCCGCTCGACGGCCTCGATGGCCTCGCCGACGGCCCCCGGGTTCTGC CCGCCGCCCTGGGCCACGTCCGGCTTGCCGCCGCCTCCGCCGCCGAGCGTCTTGGCGGCGGTGCGGACCA GGTCACCGGCCTTGAGCCCGCGCTCTCGTGCGGCCTCGTTGGTGGCGATGACGGTGAGCGGACGGCCGTT CGCCACGGTGAAGAGGGCGACGACGGCCGGGCGGTCGCCAGGGACGCGGCCACGCACGTCCAGGACCAGC TTGCGCAGATCGTCGGAGGAGGTGCCGTCCGGCACCTTGGCGGCGACCAGGGCCACGCCCCGGACGTCCT TGGCGCCCTGGACGAGACCGGCGGCGGCCTGCAGCACCTTCTCGGCGCGGAACCGCTCGATCTCCTTCTC GGCCTCCTTGAGCTTGCTCAGCATGCCGGAGATCTTCTCCGGGAGCTCCTCGGGGCGGCCCTTGACCAGG TCGGTGAGCTGGGAGACGACCGTGTGCTCCCGGGCGAGGAAGTTGTAGGCGTCCACGCCCACCAGGGCCT CGACCCGGCGCACCCCGGAGCCGATGGAGGACTCGCCGAGCAGCTTCACCAGCCCCAGCTGGGCGGTGTT GTGCACATGCGTGCCACCGCACAGCTCCTTCGAGAAGTCGCCGATCGTCACCACGCGCACCCGGTCGCCG TACTTCTCGCCGAACTCGGCGATGGCGCCCTGCTTCTTGGCGTCGTCCATGCTCATCACCTCGGCGGTGA CGTCGAGCTCGCGGGAGAGCACCTCGTTGATCTTCTGCTCGACGTCGGTGAGGACCGCGCCGGGCACGGC GGTCGGCGAGCCGAAGTCGAAGCGGAAGCGGCCGGGCGAGTTCTCCGAACCGGCCTGGGCGGCGGTCGGG CCCAGGGCGTCGCGCAGCGCCTGGTGGGTGAGGTGGGTGGCGCTGTGGGCGCGGGCGATGGCACGGCGGC GCTTGATGTCGATCCGGGCGTGGGCCCCGGCGCCGACCGTCACCTCGCCGACCTGGACGACGCCCTTGTG CACGCTGACGCCGGGCACCGGCTGCTGGACGTCCCGGACCTCCACCACGGCGCCGGTGTCCAGCTTGATC CGGCCGGTGTCGGCGAGCTGGCCGCCGCCCTCGGCGTAGAACGGGGTGCGGTCCAGGACGACCTCGACCT CGTCGCCCTCGGTGGCGGCGGGCGAGGACACCCCGTCGACCAGCAGGCCGACGACGGTGGACTCGCCCTC GGTGTGGAGGTAGCCGGTGAATTCGGTCAGGCCGGAGCCGTCGGCCACCTCGCGGTAGGCGGACAGGTCG GCATGGCCGGTCTTCTTGGCCTTGGCGTCGGCCTTGGCGCGCTCCCGCTGCTCCTTCATCAGGCGGCGGA AGCCCTCCTCGTCCACCGAGAGGCCCTGTTCGGCGGCCATCTCGAGGGTGAGGTCGATCGGGAAGCCCCA GGTGTCGTGGAGCAGGAACGCCTTGTCCCCGGGGAGCACGCTGCCACCGGCGCTCTTGGTGTCGGTGACG GCGGTGTCGAGGATGTTGGTGCCGGCCTTGAGCGTCTTGAGGAAGGCCGACTCCTCGGCGAGGGCGACGG TCTCGATGCGCTTGCGGTCGGTGAGCAGCTCCGGGTACTGCTGGCCCATCGTCTTGAGGACGACGTCGAC CAGTTCGCCGACGACCGGCTCGGTGGCGCCGAGGATGCGCATGTTGCGGATGGCGCGGCGCATGATGCGG CGCAGCACATAGCCGCGGCCCTCGTTGCCGGGGGTGACGCCGTCGCCGATGAGCATGACGGAGGTGCGCA TGTGGTCGGCGACCACGCGCAGCGAGACGTCGGAATCATGGGCGGCGCCGTAGGCGACCCCGGTCAGCTC GGTGGCCTTGTCCATGACGACGCGCAGGGTGTCGGTCTCGTACATGTTCCGCACGCCCTGGAGAATCATC GCCAGGCGCTCGAGGCCGAGGCCGGTGTCGATGTTCTTGCTCGGGAGCTCGCCCAGGATCGGGAAGTTCT CCTTGCCCTCGCCCGGACCGCGCTCGTACTGCATGAAGACCAGGTTCCAGATCTCCACGTAGCGCTCGTC GTTGACGGCCGGGCCGCCCTCCTCGCCGAACTCCGGACCGCGGTCGTAGTTGATCTCGGAGCAGGGGCCG CAGGGGCCGGGGACGCCCATGGACCAGTAGTTCGGGCCCATGCCCAGGCGCTGGATGCGTGTCGAGGGGA CGCCGATCACCTCGCGCCAGATGCGCTCGGCCTCGTCGTCCTGCTCGTAGACGGTGATCCACAGCCGCTC GGGGTCGAGGCCGTAGCCGCCCTCCTCCTGGGAGCTGGTCAGCAGCTCCCAGGCGTACTTGATGGCGCCT TCCTTGAAGTAGTCGCCGAACGAGAAGTTCCCGCACATCTGGAAGAAGGTGCCGTGGCGGGTGGTCTTGC CGACCTCTTCGATGTCCGGGGTGCGTACGCACTTCTGCACGCTGACGGCGCGGTCGAAGGGCGGCTTGAC CTCACCGAGGAAGTACGGCTTGAAGGGCACCATGCCCGCGGGGACCAGCAGCAGCGTCGGGTCGTCCGCG ATGAGCGACGCCGACGGCACGACGGTGTGCCCACGCTCCTCGAAGAAGCGCAGCCAGCGGCGGCGGATTT CAGCCGACTCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 2760285-2762957	Sequence	M271_10285 - alanyl-tRNA synthetase (Protein is coded in complementary strand; from 2762957 to 2760285)
ORF... 2762957-2763286	Sequence	M271_10290 - hypothetical protein (Protein is coded in complementary strand; from 2763286 to 2762957)