DNA sequence >NC_022785, from 23928 to 24087 (160 bp); Streptomyces rapamycinicus NRRL 5491 CTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNN Translate to protein Restriction digest BLAST Design primers with primer3