Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 2180791
End position: 2181603
Length: 813

DNA sequence
>NC_022785, from 2180790 to 2181602 (813 bp); Streptomyces rapamycinicus NRRL 5491 TCAGGGGGAGTCAGGTGCTGGGGCGGCACGGGTGAAGTCGGGCGCCCGCTTGGCCAGGAAGGCGGCGACG CCCTCACGGCCGGCGGCGGAGGCGGCCGCGGCGGCGATCGCACGGGCCTCGCGGTCCAGGTGCGTGGCGA GGCCGTCGGTCTGCCCGGCGGCGACGAGACGGCGGGTGGCGCCGAAGGCGGCGGTGGGCCCGTCGCGCAG CGCCTCGGCCATACGCAGCGCCTCGGCGGCCAGCCGGTCGGCGGGGACGACCCGGCTCACCAGTCCGATG GTCTCCGCTTCCGCCGCCGCGATACGGCGGTTGGTCAGCAGCAGGTCCATGGCCCGCTTGGGACCGACCA GCCGGGGCAGGAACCAGCTCACTCCGGCGTCCGGTGAGTAGCCGATCCGGGTATACGCGGTGGTGAACGA GGCGTTGTCGGCGGCCAGGGTCATATCGGCGGACGCGGCCAGGCCGATGCCCGCACCGGCGACGGCTCCC TGCACGGCGGCCACCACGGGCGCGTCGCCCGCCGCGAACGTGCGCAGCGCGTCGTGGAGCGCGCCGGTCA CCGCGATGAGGTGCCGCTCCAGCGGCTCTCCGGAGAGCCGGGAGAACTCCCCGAGATCGCCACCCACGCA GAAGGACTTCCCCTCGCCGGTCAGCAGCACGGCCCGGACGGACTCGGTGGCGCAGGCCCGCGCCGCGTGC AGCAGTCCGCGTGCCATGTCCAGGCCGATGGCGTTGCCCGCCGCGCCCCGGCGCAGGGTGACACGGGCCA GACCGTCGTCGATCTCGATGCCGACGCTCATCGCGCGGTCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 2180791-2181603	Sequence	M271_08550 - crotonase (Protein is coded in complementary strand; from 2181603 to 2180791)
ORF... 2181588-2182367	Sequence	M271_08555 - short-chain dehydrogenase (Protein is coded in complementary strand; from 2182367 to 2181588)