DNA sequence >NC_022785, from 2016379 to 2016538 (160 bp); Streptomyces rapamycinicus NRRL 5491 AGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNN Translate to protein Restriction digest BLAST Design primers with primer3