DNA sequence >NC_022785, from 2016376 to 2016525 (150 bp); Streptomyces rapamycinicus NRRL 5491 CGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNN Translate to protein Restriction digest BLAST Design primers with primer3