Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 1960904
End position: 1962412
Length: 1509

DNA sequence
>NC_022785, from 1960903 to 1962411 (1509 bp); Streptomyces rapamycinicus NRRL 5491 ATGAGGCAACAGACCCACAAACGCGGGCGGTTCACTAACAAGACCGTCGCCATCGTCGCCGCGCTGGCGC TCGGCGGCGGTGGGGCCGCGATCATCGCCGCCAACGCGTCCGCCCACGGCGACAAGAGCGGCTCAGAGCA GAACAACACCGTGGCCGCGAAGGCGGGGACGATCCAGTGCCCCGACGTGGGGCAGCAGCTGAGTGACGTG CCGTCGAAGGCCCAGCCCGAGGTCGACAAGAACCTGGCCGAGCTGGACAACCAGGTGGCCAAGGCGTACC AGCAGATGAACCAGCAGCAGGGTTCGAACGACGCCGTGCTCTCCAAGCTCAACGACGACCGCTCCCAGAC GCTGAACAACATCGGCTCGGCCATGGGCGGCGACAACTCCCAGCTGCAGGGCATGGCCAACTGCAAGTGG CAGGAGGTCGCCAACCAGGCCGGCCAGGGCCAGGACGGTCAACAGCAGGGCGGCCAACAGCAGGGCGGCG ACCAGCAAGGTGGTCAGCAGCAAGGCGGCGACCAGCAAGGTGGTCAGCAGGGCGGCGACCAGCAAGGTGG CGACCAGCAGGGCGGCGAGGGCGCCAACGAGGTGCCCGGCAACGCCGGCGGCCAGGCCGGCAACGGCCCG TCGCAGGACGACTTCGTCGACATCACCAAGGTGCAGCCGAACGTCCAGCAGCCGCCGGAGGCCCAGGGGA ACGGCTCCAGCGGCACCTTCTCCTCGGAGTGCGGCGTCGGCGACCCCCAGCTGCGCAACAGTGACAACGT CATCGTCGCGCCCGGTGTCGGCAACGGCGCCCACCACACCCACGACTACGTGGGCAACGACAAGAACGAC GCCTTCGCCAACAACCAGACCTTCGAGCAGGGCGGCACCTCCTGCGAGAACCAGGACGACAAGTCGACCT ACTACTGGCCCGTGGTCCGTGCCCAGGACGGCAAGGACGAGGCGGACGCCAACGAGCTCGGCGGCGGCCA GGAGGGCAACGTCGGCACGATCCTGACCCCCAAGAGCTCCAAGATCGAGTTCAAGGGCAACGCACAGAGC AAGGTCACGGCGATGCCGAAGTTCCTGCGCATCATCACCGGTGACGCCAAGGCGTTCACCAACGGCGACA AGAACGCCAACGCCTCCTGGAGCTGCACCGGCTTCGAGGACCGTCAGCTGACCGACAAGTACCCGCTCTG CCCCGAGGGCAGCTCGGTCACCCGGACCTTCGACTTCCAGAGCTGCTGGGACGGCCAGAACATCGACAGC GCCAACCACCGTGACCACGTGGCGTTCGCGGGCGAGGACGGCTCCTGCCCCAACGGCTTCAAGGCCATCC CCCAGCTGGTGAACACCATCACCTACGACGTGCCGGCCAACACCCCGTTCGCCGTCGACGGCTTCCCCGA GCAGTTGCACAAGCCCATCACGGACCACGACGACTTCATCAACGTGATGTCGGAGGACCTGATGAACGAG GCGGTGGACTGCATCAACAGCGGCAAGCAGTGCGGCTGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1960904-1962412	Sequence	M271_07445 - hypothetical protein
ORF... 1962403-1963347	Sequence	M271_07450 - RNA polymerase sigma-70 factor