Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 1945551
End position: 1946858
Length: 1308

DNA sequence
>NC_022785, from 1945550 to 1946857 (1308 bp); Streptomyces rapamycinicus NRRL 5491 TCAGCCCTCCGTCCGCAGGTCCTTGACCCGGCGGATCTTGCCCACGGAGCGCTCAAGCGTCTCCGGGTCC ACGACCTCCACCTCGACCGTGAGCCCCACCCCGTCCTTGACCCCCCGGGCGATCGCCGTGGCGGCGTCCG CGCGCCGCTCGGGGCCGGCCCCGGGGCGTGCCTCGATCCGCACCGCCATATGGTCCATCCGGCCGCGCCG GGTCAGCTCCAGCTGGAAGTGCGGCGCCACCGACGGGACCCGCAGCACGATCTCCTCGATCTGGCTGGGG AAGACATTGACCCCGCGCACGATGAGCATGTCGTCACAACGGCCGGTGATCTTCTGGATGCGCCGGAAGG CGGGGCGGGCGGTGCCGGGCAGCAGCCGGGTCAGATCCCGGGTGCGGTAGCGGATGACGGGGAGCGCCTC CTTGGTGAGGGTGGTGAAGGTCAGCTCGCCCTCCTCGCCGCCGGGCAGCACCTCGTCGGTGAGCGGGTCC ACGATCTCGGGATAGAAGTGGTCCTCCCAGACGTGCAGCCCGTCCTTGGTCTCCACGCACTCCTGGGCCA CCCCCGGGCCCACCACCTCCGAGAGCCCGTAGATGTCCACCGCGTGGATGTCCATGCGCTCCTCTATCTC CCGGCGCATCTCCTCCGTCCAGGGCTCGGCACCGAAGATGCCGACCTGGAGGGAGGTGGAACGGGGATCC ACGCCCTGCCGCTCGAACTCGTCCAGGAGCGCCAGCAGATAGGAGGGCGTGATCATGATGATCTCGGGCC TGAGGTCCTGGATGAGCCGCACCTGGCGGGCGGTCATCCCCCCGGAGGCGGGGATGACGGTGCAGCCGAG CCGCTCGGCGCCGTAGTGGGCGCCGAGGCCGCCGGTGAACAGGCCGTAGCCGTAGGAGATGTGCACCTTG TGGCCGGGGCGGCCGCCGGCCGCGCGGATCGAGCGGGCGATCACCTCCGCCCAGACCGACAGGTCGTGCT CGGTGTAGCCGACGAGCGTGGGGCGGCCGGTGGTGCCACTGGAGGCGTGGAGCCGCCGCACCTCGCTCTG CTCGACGGCGAACATCCCGAAGGGGTAGCTGTCACGCAGATCGGCCTTGGTGGTGAAGGGGAACCGGGCC AGGTCCTCCAGGGTGCGGCAGTCCTCGGGGCCGACCCCGGCCGCACGGAACTTCTTGCGGTACACCTCGA CATGGTCGTACGCATGGCGCAAGGTGGCGCGCAGCCGGGTGAGCTGAAGCGCCCTCAGCTCCTCGGGGCT CATCCGCTCGGCGTCGTCGAGCGGAGCCGCGCTCAGCGATTCCACCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1945551-1946858	Sequence	M271_07380 - phenylacetate-CoA ligase (Protein is coded in complementary strand; from 1946858 to 1945551)