Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 1436608
End position: 1440261
Length: 3654

DNA sequence
>NC_022785, from 1436607 to 1440260 (3654 bp); Streptomyces rapamycinicus NRRL 5491 GTGGGCGTGGCCGCCGTCAACGGACCCGGTTCCGTGGTGGTCTCAGGGCCCCCGGACCAGGTCGCCCACG CGGTCACCGCCTGCCAACAAGCAGGCCGCCGCGCCCGGCTCATCGACGTCGACTACGCCTCCCACAGCGT CCAAGTAGACGAAATCGCCCATGAGTTGAACGAACTACTCGCCGGGGTCAAGCCGGTGCCGGGCCAGGTG GCGTTCTACTCCACCGTCACCGGCACCCGCATGGACACCAGCGGACTCGACACCGCGTACTGGGTCAAGA ACCTCCGCGAACGAGTGCGCTTCGCCGACGCCGTACGAGCACTCCTCGACGACGGCCACCGCGTGTTCAT CGAAGCCAGCACCCACCCCGTCCTCACCATCGGCATGCAGGAGACCATCGAGGAGGCGGGGGTCGACGCG GTCACCATCCCGACCCTGCGACGCGACCACGGTGGCGCGGCCCAGCTGGCGCGGTCGGTGGCGCAGGCGT TCACCGCCGGAGTCACGGTCGACTGGACCCGCTGGTTCCCAACCGACCCCGCGCCCCGTGTCATCGACCT GCCCACCTACGCCTTCCAGCGTGAGCGGTACTGGCTGGACGGAGAGGGCGGGCCGGGTGGCGATCCGGCC GATCTGGGGCTGACGGCCGCCGGGCATCCACTGCTCGGCGCCGCCGTGGAACTCGCCGACGGCACCACTC ACGTGCTCACCGGCCGGATCTCCACGCGGTCCCATCCATGGCTCGCCGAGCATGTGGTGGCGGGTACGGT GCTGGCGCCGGGCGCGATGTTGGTGGAGTGGGCGCTGCGAGCGGCAGACGACGTCGGCTGCGGCGGGGTG GAGGAGCTGGCGCTGCAGGTGCCGCTGGCGCTGCCCGAGTCCGGTGGGCTGCGGGTGCAGGTGGTGGTGG GTGCCGCGGCCGAGGACGGGCGGCGTGAGGTGCGGATGTACTCGCGTCCCGACCGCGCCGACCGGCCCGA CCGACCCGACGGCTCCAACGGCGTCGACCACGCCCCCGCCCTCGATTCCGGCCCGGACAGGGGGTGGGTG TGTCATGCGGTGGGCGTGCTCGCGCCCGCCGCCGACGCACCCGCACCGGTGGAGGGTCTGGGCGGGGCAT GGCCGCCGCCGGGCGCGGAACCGCTGGACGTCGGCGCCTTCTACGAGCAGGTGATGGCGGCGGGGTACGG CTATGGCCCGGCCTACCAGGGCCTGACGGCCGCGTGGCGTCAGGGCGGGGATGTCCTCGCCGAGGTGGCG CTGCCCGATGCCGCGGGAGGGCGGGACGGATTCGGCATCCATCCGGTGTTGCTGGACGCGGCGCTGCATC CGTCGCTGCTGATGGACCGGCCCGAGGGCCAGGAGGAGGACGGCCAGGTGTGGTTGCCGTTCGCCTGGAA CGGCGTGTCACTGTGGGCCACCGAGGCGGCCACGGTACGGGTCCGGCTGTCGCGGGACGAGGAGCGGCAG TCGTTGCGGCTGACCGTGGCGGACACGGTGGGCACGCCGGTGCTGACCGTCGACTCGTTGGTGACACGTC CAGCGGCGACGGACCGGTTGCGCACGGCCGCCCGCCCCGTGGACGGGCTGTTCACCCTGGACTGGACGCC CCTGCCCGCTCCGGCCACCGCGCCGTCCCCCACCTCGGTGGTCGGCGACGGCGGTTGGGTGGTGCTGGGC GAGGACCGGCTGGGGCTGGCGGAGCCGGTCCGGGCGGGCACGAGCGATGCCGTGGTGGGCCATCCGGACC TGGAGGCGCTGATCGCGGCCGTCGACGCGGGCGAGCCCGCGCCTGCCGTCGTCCTGGCCCATCCGTACGC CGCCGAGAACGGCCACGGGGACGGACTGGAGGCGACCGAGGAGCTGCTGGGGCTGGTGCAGTCCTGGCTG CGGCGCCCGGCGCTGGCCGACACGCGGCTGGTGGTCGTCACGCGGGGCGCGGTGGCAGCGGCCCGTCCCG ACAAGGACACCGATGACACCGCACTGGATCCGTGCGGTGCCGGGGCATGGGGGCTGATCCGCAGCGCCCA GTCGGAGAACCCAGGCCGGTTTGTACTGCTCGACCTCGCTGAGAGCCACGAGGGCCACACGGACCGCGAG GTCCACACGGACCACGAGAGCCACACGGACCACGAGAGCCTCGTGCAGGCCGTGGTGCGCGCGATCGAGG CGCATGAGCCCCAGGTGGCGGTGCGGGGCGGACGGGTGCTGGTGCCCCGGATGGTGCGCGCGACGGCCGC GACCGGCCCGGCCATCGATGGCCAGGACGCGGAGGAGACCGCCGGGCTCGACACCGACGGCACCGTGCTG ATCACGGGTGGCACCGGCACCCTGGGCGCTCTGGTCGCCGAACACCTGGTGCGCACATGGGGCGTCAGGC ATCTGCTGCTGGTGAGCCGGAGCGGTCCGGAGGCCGCCGGCGCAGATGAACTCACCTACCGACTCGCCGA GTTGGGGGCGCGGGTACACATCGCCGCAGCCGATGTCACCGACCCGGACGCGGTGGCCGATCTGGTGGCC GGGGTCGATCCCGCACATCCGCTGACCGGTGTGGTCCACGCGGCCGGGGTGCTGGACGACGCCGTCCTCA CCACCCAGACCCCGGAGCGGCTGGCGCGGGTGTGGCGTCCGAAGGCCACGGCCGCGGCCCATCTGCACGC CGCGACCGCGGGGCTGCCGCTGTCCCTGTTTGTGATGTTCTCCTCCACGGCGGGCACGCTGGGCGCCTCC GGGCAGTCCAACTACGCAGCGGCGAACGCCTATTGCGACGTCCTGGCCGCCCGGCGCCGCGCCCTCGGCC TGCCGGGGCTTTCGGTGGCGTGGGGCCTGTGGGCGGACGCCAGCGGGATGACCGGGCACCTGGGGGAGAC GGACCGGGCCAGGATGAGCCGGTCGGGCATCGGGGCGATGTCCAGCGAGCGGGCGCTGGGGCTGCTGGAC GCCGCCGTGCGCCACGGCCACCACCATCTGATCGCCATCGACCTGGACGTACGGGCCCTGGCGGGCCGGC CGACGCTCACCCTGCCCGCTCCCCTGCGGGCGCTCACCGGTGGCGGTACGGCCCGGCGCACCGCGGCCAC GGCCCGGCCGCACACCGACTGGGCCGGTCATCTGGCCGCGCTGCCGGTGGAGGAGCAGCGCCGTACGCTG CTCAATCTGGTCCTCACCCATGCCGCGGCGGCGCTGGGCCACTCCGACCCGGGGCGGATCCAGACCGAAC GCGGCTTCCTGGAGCTCGGCTTCGACTCGCTCACCGCGATCGAACTGCGCAACAGGCTGACGGCCGTGAC CGGGCTGCGGCTGGCCACCACCCTCATTTTCGACCACCCGAATCCGGCCGCGCTCGCCGACCATCTGCAT GCGGAATTGGCGCCCGGGGAGGTGGATCCGCTCGCCCCCATGCTGGGTGAAATCGACAGGCTGGAAAGCG CGCTGCTTTCCGTGGCCCGGGACGAGACGGCGCATGAGGCGCTGTTGAAGCGCCTTCAGTCCACGCTGTC GAAACTGGGGGCCCTGCACGGCGGCGGGACGGAGGAAGGCGAAGCGGTCGGCCGTATTCAGGACGCCACG GCCGACGAGATCTTCCAGTTCATCGACCAGGACCTGGGCCGAAGCGAAAGCGACGGAGAGCATGCACATG GGGGCACACGGTGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1436608-1440261	Sequence	M271_05595 - hypothetical protein
ORF... 1440258-1440695	Sequence	M271_05600 - hypothetical protein