DNA sequence >NC_022785, from 1360975 to 1361134 (160 bp); Streptomyces rapamycinicus NRRL 5491 GCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNN Translate to protein Restriction digest BLAST Design primers with primer3