DNA sequence >NC_022785, from 1360972 to 1361121 (150 bp); Streptomyces rapamycinicus NRRL 5491 ATGGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNN Translate to protein Restriction digest BLAST Design primers with primer3