Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 1285559
End position: 1288369
Length: 2811

DNA sequence
>NC_022785, from 1285558 to 1288368 (2811 bp); Streptomyces rapamycinicus NRRL 5491 TCACGAATGCATGCGAGGCAGCAGCTCGATGAGCTCGCGCCGCCCGGAGACGCCCAGCTTGCGATAGGAA CTCGTCAGATGCTTCTCGATGGCGCGGGAGCTCACCCCCAGCTCGGCCGCGACCTCCTGGTTGGTCAGGC CGCGTCCGACGAGGGAGGTCACGGTGCGTTCGGTACGGGTGAGGGTCGCCTCCGGCGGGGCGGCCGCCGC ACCGCTGCCGTCCGCCGCGCGGATCCGCTCGACCAGCCGTGGCACCCCGCACGCCGAGGCCAGCGCCGCG GCCTCCCGGAGTACCGCCGCCGCCTCGCCGCCTCCCGGCCGCAGCCGCTTGCCCAGCAGCAGGAGCGTGC GGGCGAGTTCCAGCTCATTGGCAGAGGCGCGCAGCACATCGACGGATTCGTGCAGCAGCCGCAGCCCCTG GTCACCGTCGCTCAGCCGGGCCTTCGCCCGCAGCGCGCGGCCGAGCGCCACCGGGGCGCCCCATTCCGTC GCCCGCAGCAGCTCCTCCTCGGCCAGCGCCCCGGCCGCGTGGGGGTCGCCGACCCGCCGGTGGAGGTCGG CCGCCCAGGGCCGCCAGGAGAAGAGCACGGGATTGCGCCAGCCCGACGCCTCCAGCTGCCGGCCGCAGGC CAGGAGGGTTTCCAGGGCGCTGGTCCAGCGGCCCTGCTCGGCGTCCTGCGCCGCCTTGACGAACTGCAGC GCCGCGGTCAGGGCCAGGCTCGAGGAGCGGCGCCGGCCGGGGCCGTCCAGAATCCGCTCGATCAGCGCTG AGTCCTGGAGGTCCAGGGCGACGCGGGTGAGGGCGAGGGTGGAGACGACCCCGGCCTCCTGCCAGTGCAC CTCGGCCAGCCGCACGGCGCGCTCGGCGTACTCACGGGCCTGGGCGACGCGGCCGCGGGCGCCGAGCACC ATGGCCTGCTGGACGTTGACGAGCGCGTCCACGACCGTGCCGCCCTGCCGACGCGTCTGCTGCTCCATCG CCAGCCAGGAGCTGATCCCCCGTACCGAATCGGCCGCCACCAGCCCGATCACCACCAGCGGCAGCGGGGT GTGGACCTGGTCGGAGGTGGCCGGTTCGCGCTCCAGGATGCGGTTGGCCAGCCGGGAGACCTCGGCCGCG GGCCATCGGGCGGTGAGCACCGCCGCGTTGAGCAGCACCGCCGTCAGCTCGCGCTCGGCGGAGGTCATCA GCGGCGGCTCCTCCCCCAGCGCGGACAGCCGCTCGACCGCCGCGGCCAGCTCAGCCGGGTCCTCGTGTCC GGCGTGCCGCAGCCGCGCCTCGAGCCGCAGCGCCACATCGCGCGCCGTGCCCTCCAGCGAGGCGGCGGGC CCCAGGTCCTCGGCGACCTGGTGCAGCAGATCGATGACCGACGGGGGCCCCAGGCCCACGATGGTGGGCG AGATCCGCAGGGCCGCGGCGGCCCGGAGCCTGGCCGTGGTCAGCAGGGGTATCGCCTGGGAGATGTGGCG TTCACAGGCGGCCGGGTCGAAGCCGCGCTCCGCGGTGGCCAGGTCGATCAGCAGCCGGGCCCGGCCCTCC CCGGTCAGCGAGCCGTCCAGGAGGGCGCGGCGCAGAAAGCGGGCGGCGGTGCGGGGCGCGCCGCGGCGCA GCGCGGTGTCCGCCGCGGCGCGCAGCACCTCCACCGCCCAGGGGTGGTCGGAGCTGGTGACGGCCAAGAG CTGGGCGGCGACGAGTTCGGCGGGGCGTCCGTTGTGGTACATCAGGGCCGCCGCGGCGTCGTGCAGCCGC TCCCGTTCGGCCACGGTCATCGAGGACTCGACGGCGTCCTGCACCACCCGGTGGAGGAAGCGCGGCTCCT GCTCGGCGGCGAGCAGCCCCAGCTGGTGGAGGTCGCGCAGCGCGGCCAGGTAGCCGATCGGGTCCAGCCT GGCCAGCCGCCCCAGCAGCTCCGGTTCGGCCTGGTCGCCGAGGACCGCGATGGCCGCGGCCAGGTCACGC GGGGGCCGGGGCTGGGCGCGCAGGGAGCTGGCCAGCCGCTCGCGCAGCTGGGCGGGGCGCAAGGAGCGGG CCGATTCGACATGCTCGGCGGTGGGCCGGTGGCCGCGCATGGCCAGGTCCGCCAGCACCGACTTCAGGAA CAGCGGGTTCCCCGCGGAGCTCTCGTGGCAGGCACGGGCGAACTCCTCGTCGCCCGGCACGCCGAACTGC TCGAGGATCACTTCCTTGGTGGCGCCCAGCGAAAGCGGCGCGGGGCGCAGCACCCGCCGCGCCGCCTCGA CGATCTCCGGCAGCAGTGGGTCCCCGGGGCGCCGGTAGCCGTCCCGCAGCGCGCAGACGACCACGGCGCG CAGCCCGTGCAGCCGCTTGACGAGGAAGGCGAGCCAGCGCAGCGAGGGGGTGTCGACCCACTGCAGATCG TCCACGAGGATGAGCAGCGGGCGTTCGGCGCCGGCGGTGGCCAGTACCGAACGCAGGCCGTACAGCGCTT CCTCGGGGATGGTGACGGCTCCGTCCTCGTCGATCGGCGGGTCGTCGTCGGAGAAGACCGTCCGGCAGAG GTCGACCTCTGTCTCGATGCGCTGCCCGCGGAGTTCTTCCGGGGCGCCGGTGAGCAGGCTGTCGAAGAGC TGGCGGACCACACCGAGGTCGAAGTCCTGCTCCATCGGCGCGGCGTTGGCGCGCAGGACCCTGATCTTCT CGCCCTCGGCGAGCATCGGCAGCTGGTGCAGCAGCGCGGTCCGGCCGGTGCCCAGGGGGCCGGTGAGCAA CAGCAGTGACGAGTCTCCCGCCGCCGCGGCCCGCAGGGCCTCCGCGACCTGCGCCAGCTCGGCCTCCCGT TCAAGCAACAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1285559-1288369	Sequence	M271_05080 - LuxR family transcriptional regulator (Protein is coded in complementary strand; from 1288369 to 1285559)
ORF... 1288359-1291115	Sequence	M271_05085 - hypothetical protein (Protein is coded in complementary strand; from 1291115 to 1288359)