Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 1249887
End position: 1251092
Length: 1206

DNA sequence
>NC_022785, from 1249886 to 1251091 (1206 bp); Streptomyces rapamycinicus NRRL 5491 TCATCGGGGCAGGTCCTTCCACGGGACGTCCTTGTCGGTCCGCGGCTCGGGCGGCAGGCCCAGGACGCGT TCGGCGATGATGTTGAGCAGCACCTCCGAGGTGCCGCCCTCGATGGAGTTGCCCTTGGCGCGCAGATAGC GATAGCCGGCCTCGCGCCCGGTGAAGTCCACGAGTTCGGGGCGGCGCAGCGTCCAGTCGTCGTAGAGCAG CCCCTCCTCCCCCAGCAGCTCGACCTCCAGCGCGCTGATCCGCTGGTTGAGGCGGGCGAAGGCGAGCTTC ATGGCCGAGCCCTCGGGGCCGGGCTGGCCGACCGCGAGCTGCTGGCGCAGCCGCTCGCCCGCGAGCCGGG CGGCCTCGGACTCCGTCCACAGCCGCAGGAGCCGCTGGTGGAGGTCGTGGGTGCGCAGTTCGGGCCGCTC GCGCCAGGTGTCGGAGACGAGGCCGATCATGCCGCCCTCGCGGGGGACGCGGCCGCCGCCGATGGCGACC CGCTCGTTCATCAGGGTGGTCTGGGCGACCCGCCAGCCCTCGCCGATCTCGCCGAGCCGCCGGCTGTCGG GGATCCGCACACCGGTGAGGAAGACCTCGTTGAACTCCGCCTCGCCGGTGATCTGGCGCAGTGGCCGGAC CTCGACTCCGGGATCGGTCATATCGCAGATGAAGTAGCTGATGCCGCGGTGTTTGGGCTGGTCGGGGTCG GTGCGGGCGATGAGGATGGCCCAGCGGGCGAGATGGGCGCTGGAGGTCCAGACCTTCTGTCCGTCGACCA CCCAGTGGTCGCCGTCGCGCACCGCCCGGGTGGCGAGCGCCGCGAGGTCGGATCCGGCGCCGGGTTCGCT GAACAGCTGGCACCACACCTCCTCGCCGGTCCACAGCGGGCGCAGCAGCCGCTCCCGCTGCTCGTCGGTG CCGAAGCCGAGGATGGTGGGGGCGGCCATGCCGAGGCCGATGCCGATGCGGCGGGGGTCGTTGCCGGGCG CCCCCGCGGCCTCCAGCTCGGCGTCGACGACGGCCTGAAGGGAGCGCGGGGCGTCCAGGCCGCCGAGTCC GGCCGGGTAGTGCACCCAGGCGAGCCCGGCGTCGAAGCGGGCGCGCAGGAAGTCCAGGCGGTCGGTGGTG GCGGGCGGATGGGCGTCCAGGAGTTCCCGGACGCGGCGGCGCAGTTCGCCGGCGTCCACGGCGGGGCGGT GCCGGGTGGTGGTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1248739-1249890	Sequence	M271_04945 - acyl-CoA dehydrogenase (Protein is coded in complementary strand; from 1249890 to 1248739)
ORF.. 1249887-1251092	Sequence	M271_04950 - acyl-CoA dehydrogenase (Protein is coded in complementary strand; from 1251092 to 1249887)
ORF... 1251089-1252093	Sequence	M271_04955 - alcohol dehydrogenase (Protein is coded in complementary strand; from 1252093 to 1251089)