Sequence retrieval

Genome: Burkholderia pseudomallei NCTC 13179
Start position: 859309
End position: 860058
Length: 750

DNA sequence
>NC_022659, from 859308 to 860057 (750 bp); Burkholderia pseudomallei NCTC 13179 TCATTCGGCGTCCTCCGTGCCCGCCGCGCGGATGCGCCGCCAGTCGGCGCGCTCGACGAACCAGTCGGCG GCGCTGCCTGCCGCGAGCGCCGCGACCACCGCGAGCGGCAGCGCGAGCCGGTACGGCAGATCGAACGCGA CGAGCGACACGATCCCCGCCACCGCGACGGCCGCGAGCGTCGAGCGGTTCGAGATCGCCGAGACCATGAT CGGAATCAGCGCGAGCGTGCCGGCGAGCGCGAGCCCCCAACTGTCGGGAAACGCGCTCGCGAGCGCGATG CCGACGAGCGACGAAGCCTGCCACGCGAACCAGCTCGTGAGCGCCATCCCCCAGAAGTACGCCTCCTTGC CGGGCACGTGGCCCGCGGCGAAGCCCTGCTTCTGGAACAGCAGATAGATCACGTCGCCGTTGAAATAGCC GATCGCGAGGCGCCGCCACAGCGGCAGATACGAGAAATGGGGTGCGAGCCCCGCGCTGAAGATCACGAAG CGCATGTTCACCATCGCGGCCGTCAGGAGAATCGTCCAGACCGGCAGCTTCGCGGCGAACAGCGGCAGCA CGGCGAGCTGCGACGAGCCCGCGTAGACGAGGATCGACATGCCGAGCGCCTGCCCCGTCGTCAGCACCGA TTTGCTCATTGCGATGCCGGTGACGAGCCCCCACGACAGGATCGCCATCAGCGTCGGGGAAAAGTCGCGG AAACCCTGCGCGAGGGCGAAACGGTCGGTGGCGGACAGGCGTGCGAGCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 858989-859312	Sequence	BBK_712 - branched-chain amino acid transport family protein (Protein is coded in complementary strand; from 859312 to 858989)
ORF.. 859309-860058	Sequence	BBK_713 - azlC family protein (Protein is coded in complementary strand; from 860058 to 859309)