Sequence retrieval

Genome: Burkholderia pseudomallei NCTC 13179
Start position: 54495
End position: 55199
Length: 705

DNA sequence
>NC_022659, from 54494 to 55198 (705 bp); Burkholderia pseudomallei NCTC 13179 TCAGCCGACACTGAGCCAACGCTCGACGATGCCCGCATCGAGCGCCTTCGCCGCGCCGTCGTAGACGATC CTCCCCCGCCCCATCACCGCGACCCGCGACGTCAGCGCCGGCGCGAGCTGCATCCGCTGCTCGATCAGCA GCAGCGCGACGCCTTGCGCCTTCGCGCCGGCGAGACACGCGCGAACCTGCTCGACCGCGAGCGGCGCGAG CCCCTCGGCCGGCTCGTCGACGATCAGCACGCGCGGCGCGCCGATCAACGCGCGCGCGAGCGCGAGCATC TGCTGCTCGCCGCCCGAGAGCACGCCCGCCTTCGTCCGCCAGCGCTCGGCGAGCATCGGATAGCGCTCGC GCGCGTCGGCGAGCGCCGCGCGCGCGGCGGCGCCCGACGCGCCGCGCAGGCCGAGGCGCAGGTTGTCCTC GACGCTCAGGAGCGCGAACACGTCGCGATGCTCGTCGACGTAGCCGACGCGGTGCCGCGCAATCGCGAAC GTCGGCGCGCCCGCGACATCGACGCCGCCGATGCGCACCGTCCCCTCGACCCGCACCATGCCCATGATCG CCTTCGCGAGCGTCGAGCGGCCCGAGCCGTTGCGGCCGACGAGCGCGACAGCCTCGCCCGCCGCGATCCG CAAATCGACGCCGTCGAGCACCGGCAGCGCGCCGTAGCCGGCGCGCACGCCGCGCAATTCGAGCAGCGCG CTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 54495-55199	Sequence	BBK_44 - ABC transporter family protein (Protein is coded in complementary strand; from 55199 to 54495)
ORF... 55196-57454	Sequence	BBK_45 - branched-chain amino acid transport system / permease component family protein (Protein is coded in complementary strand; from 57454 to 55196)