Sequence retrieval

Genome: Burkholderia pseudomallei NCTC 13179
Start position: 450397
End position: 451167
Length: 771

DNA sequence
>NC_022659, from 450396 to 451166 (771 bp); Burkholderia pseudomallei NCTC 13179 ATGAAGGCGGCACGGGAATGGACGGACGGCGGGGCCGCTCCCGGACGGCGCGGCGAGCACCGGGCGAGAT CGGTTGGGTCAGGGGGGCGGTGGGTCGACGGTACAGCAGTTCAGCAGTTCAGCAGTTCAGCAGTTCAGCA GTTCAGCCGTTCAACCGTTCAGCCGTCCAGCGATTCAGCGATTCAGCGATTCAGCGATTCAGCGATTCAG CGATTCAGCGATTCAGCGAAAAATAATCGCATGGCACGGACGCACCGCCGTTCGACCCACGCGAAAACGC CGCCGGCACGCGGCGAGGCTCGTCCGAAATGTGGTTGCGATGCCGGCAAGGACCGAGCGTGCATCGGGGC GTTCGCGACCGCGATGTCCGCCGCTCGCCGGCTCGTGCGCAAGATTGCCGACGCGACGTCGCAACGCGAC GAAGCCGCGCCGCCGCAACGTCTTCGGTCTGCCGAGGCGCGCGTGAAGTCAAGCCCGCCCTGCGACGGCG GCGCGCCGCCGTATCACCCAGGGGCCGGCGCCCGAGATGCGGCACGGGCAAAACCGCGAGCCGGCGGCGC ACGCGAAACGATAGCGCCCGCCGCCGCCCGCGCGGCTTGCACCGTCGCGCCGCCCCTGCCTTCGTTCCGG CCGGGCGCCCGCGCGCGGCGTGCCGCGCTCACGCGTTCGCGGTCAGCCGGCGCGCCAGATCGTCGAGCAG CACCCGCGCCGTCGCGACGTTGGTGGCCACCGGCACGTCGTGCACGTCGCACGCGCGCACGAGCGCGGTG A

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 450397-451167	Sequence	BBK_367 - hypothetical protein
ORF... 451055-451447	Sequence	BBK_366 mgsA MGSA: methylglyoxal synthase (Protein is coded in complementary strand; from 451447 to 451055)