Sequence retrieval

Genome: Burkholderia pseudomallei NCTC 13179
Start position: 431731
End position: 433125
Length: 1395

DNA sequence
>NC_022659, from 431730 to 433124 (1395 bp); Burkholderia pseudomallei NCTC 13179 TCAGCGCGGCGGCGCCGGCCAGCGGGGCATCTCGCCGCGCCAGGCCTCGTACGCGCGACCGAGCTGCAGC ACGAGCCGATCGGCAAAACGCGGCCCGACGATCTGCAGGCCGATTGGCATCCCGTCGCGCGCGAAGCCGC AGTTGATCGACAGCGCCGGCTGCTCGCCCATGTTCCACGGAACCGTGAACGCGATGTGCTCGAACGGCCG CGCAGGATCATCGGTCGGCCCCGCCCATTCGGCCGGAAAGCCGGTGACGACGTTGGTCGGCGACAGCACC GCGTCGACCGACTGGAACAGGCGTGCGGCGGCCGCGCGCATCTGGAACGTCGCGCCGAAGCCGCGCACCG CCTCGACGCCCGACACCGACGCGCCCTGCCCCGCCCATTCGACGATGTACGGCAGGATGCGCGCGCGCGC CGCGTCCGGCAGCCGTTCGAGGTCCGCCCATAGCCGCGCCCGCCAGAAGCGGTCGAGCCCCTCGAGCATC GGCCGCGACAGCACGGGCGGCGCGGCGACGATCCGCGCGCCGTGCGCGGCGAAACGCTCGGCCGCCGCCT CCACCGCGGCGACGATCTGCGGATCCGGCGCGAGTCCGCAGCCGGCGTCGAGCATCAGGCCGATCCGCAT GCCCTTGACGTGCGCGGGCGCGATCGACCAGTCGATCGTCTCGGGCGGCAGGCTCGTCGCGTCGCGCCAG TCCGGCCGCGACAGGAAGCGCATCAGCAGCGCCGCATCGTCGATCGTGCGCGTCATCGGCCCCGCGCAGC GGCCCGTGTAGTACGGCTCGATCGGAATCCGCCCGTTCGACGGCTTGAAGCCGACGATCCCGCACCAGCC GGCCGGCAGCCGGATCGACCCGCCGATGTCGGTGCCGACGTGCAGCGGCCCGTAGCCCGCGGCGGCGGCC GCCGCCGCGCCGCTGCTCGATCCGCCCGGATTCAGATCGAGCCGCCACGGGTTGCGCGTGATGCCGTACA GGCTCGACAGCCCCGACGACAGCATCGCGTAATCGGGCACCGTCGTCTTGCCGATCACGACCGCGCCCGC TTCGCGCAGCCGCGCCGCGGCGGGCGCGTCGACGGTCGCGGGCGGCGCGTCGGCGGCGGCCGCCGAGCCT TGCGCCACCCGGTCGCCCTGCGTCGCGATCAGTTCCTTGATCGTCACCGGCACGCCGTCGATCTCGCTGA GCGGCGCGCCCGCCGCCCAGCGGCGCGTCGACGCGAGCGCCTCGTCACGCACGCGCGCCGGATCGAAGCG GCACAGCGCATTCAGATGCGGCTCCCAACGGGCAATGTGGTCGAGCAGCGCGTGCGCGTAATCGACGGGC GTCACGCTGCGGTTCTTGAACCGCTCGGCAAGCGAGCAAGCGCTCAGGTCGAGCAACTCGGACAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 431731-433125	Sequence	BBK_351 - amidase family protein (Protein is coded in complementary strand; from 433125 to 431731)