Sequence retrieval

Genome: Burkholderia pseudomallei NCTC 13179
Start position: 3516264
End position: 3519434
Length: 3171

DNA sequence
>NC_022659, from 3516263 to 3519433 (3171 bp); Burkholderia pseudomallei NCTC 13179 TCAGGCTCCGGAAGGCGTGGCGGGCGGCTCCGGATCGCGCTCGTCCGGCTTCACGCGGAACCACGCGGCA TAGAGCGCCGGCAGGAAGAAGAGCGTGAGCACCGTCGCGCTCGTGATGCCGCCCATCAGCGCGGTCGCCA TCGGGCCGAAGAAGTTCGAGCGCAACAGCGGAATCAGCGCGAGCACGGCGGCCGCGGCCGTCAGCGTGAT CGGCCGGAAGCGCCGCACGGTCGCGCCGACGATCGCGTCGAAGCGGCCGTGGCCGGCGGCGATGTCCTGC TCGATCTGGTCGACGAGAATCACCGAGTTGCGCATGATGATCCCGAACATCGCGATCACGCCGAGCATCG CGACGAAGCCGAACGGCTTGCCGAACAGGAGCAGCGTGCCGACGACGCCGATCATCCCGAGCGGCGCGGT CAGCACGACCATCAGCACGCGCGAGAAGCTCTGCAGCTGAATCATCAGGAGCGTGAGCACGGCGATCACC ATCAGCGGCATCTGCGCGTTGATCGACGTCTGGCCCTTCGTGCTTTCCTCGACCGAGCCGCCGATCTCGA TCCGGTAGCCGACGGGCAACTGCGCGCGCAGCGCGTCGAGCTTCGCGTCGATCGCGTGCGTGACGTCGAT GCCTTGCGCGCCCGCCGTCACGTCGGACTGCACGGTGATCGTCGGCTGGCGGTCGCGTTCCCAGACGACG CCGTATTCGAGCGTGTCGTGAAAGCGCCCGAGCGAGCCGAGCGGCACCGGGCCGTTCGGCGTCGGCATCG CGAGGCCCGCGAGGCTCGCGGGATCGACGCGCTGCGCGCGCGGCGCGCGCAGATCCACCGCGATCAGCTT GTCGCGCTCGCGATACTGCGTGAGCGTCGTGCCGGAGAGCGTCATCGCGAGGAAGCTCGCGACGTCCTGC GAACTCACGTTCAGCTCGCGCGCCTTGTGCTGGTCGAGCTCGAAGCGCACCGAACGCTCGGCGGGTTCGT CCCAGTCGAACTGCACGTTGGTCGCGCGCGCATCCGCGCGCATCGTCGCCGCGACCTTCTCGGCGATCGC GCGCACGGTCGCGATGCTGTCGCCGCTCACGCGGAACTGCACCGGATAGCCGACGGGCGGGCCGTTCTCG AGCCGCGAGATCCGCGTGCGCGCCGCCGGGAATTGCTCGCGCAGCACGGGCGCGAGCCAGGCCGAGAGCT TTTCGCGCGCGTCGACCGATTTCGCGGTGATCACGAACTGCGCGAAGTTCGGCAATTGCAGCTGCTGGTC GAGCGGCAGGTAGAAGCGCGGCGCGCCGGAGCCGACGAAGTTCACCGCATGGTCGATCTCCGGGCGCTTC GCGATCAGCTTCTCGAGGCGCTCGGCCTCCTTGAGCGTCGCGTTGAACGACGCGCCCTCGGGCAGCCGCA GGTCGACGAGCAGCTCGGGGCGGTCGGAGCTCGGGAAGAATTGCTGCGGCACGAGCGAGAAGCCCGCGAG CGCGACGACGAACAGCGCGATCGTGATCGCGAGCACGATGAAGCGCCGCTCGATGCACCACTTGATCCAT ACGCGCAGCCGCGTGTAGAAGCGCGTGTCATAGATGTCATGGCCGTGCGCGTGATCGTGCGCGGCGTCGG GGGCGTGCGGCGCGCCGGCGGCGTCCTGCCGCGGATGCTTGCGCTCGGGCAGCATGTGATAGCCGAGCAG CGGAATCAGCACGACGGCCGCGAACCACGACGCGATCAGCGCGATCGCCGATACCTCGAAGATCGAGCGC GTGTATTCGCCCGTGCTCGATTTCGCGAGCGCGATCGGCAGGAAGCCGGACACCGTGACGAGCGTGCCCG TGAGCATCGGGAACGCGGTGCTCGTATAGGCGAACGCGGCGGCGCGCGCGCGGCTGAAGCCCTGCTCGAG CTTCACGGCCATCATCTCGACCGCGATGATCGCGTCGTCGACGAGCAGGCCGAGCGCGAGCACGAGCGTG CCGAGCGAGACCTTGTGCAGCCCGATGTCGAACAGGTACATGAAGAGCGCGGTGACGGCGAGCACGACGG GAATCGAGATCACGACGACCATCCCGGTGCGCAGGCCGAGCGACACGAGGCTCACGATCAGCACGATCGC GACCGCCTCGGCGACCGCCTCGAGGAAGTCGTCGACCGAGCGCGCGACCGCGTGCGGCATGCTCGATACT TCGGTGAGCGCGAGGCCGGCCGGCAGTTGCGCCTGCAGCGCCTTCGCGCTCGCGTCGAGCGCCTTGCCGA GCCGGATCACGTCGCCGCCCGGCTGCATCGTGACGCCGATGCCGAGCACGGCGCGGCCGTTCGTGTCCCG GCCGATCGTGCGCATCTGCGTGACGGGCGGATCGTCGTAGCCGCGCTTGATGGTCGCGAGCTCGCCTAAG CGGAACGTGCGGCCGTTCACGCGGATCAGCGTGTCGGCGATCGCGGCGACGCTCTCGTACTGGCCGCTCG GGCGGATGAACACGCGATCGTGCGCGGCCGTGAGCACGCCCGGCGACGCGACGTCGTTCTGCGCGTTGAT CGCCTGCGCGATCTGCTGCGGCGAGATGCCGAGCCGCGCGAGGCGCGTGTTGTCGATCTCGACGAAGATC CGCTGATCGGGGTCGCCGAAGTAGTCGACCTTCGCGACGCCCGGCACGCGCAGCAGCACGACGCGCAGCT GATCCGCGTAGTCGTGCAGTTGCGCGGGCGAGAAGCCGTCGCCTTCGAGCGTGTAGATGTTCGTGTAGAC GTCGCCGAATTCGTCGTTGAAGAACGGGCCCTGGACGCCGGGCGGTAGCGTCATCGAGATGTCGCCCACC TTCTTGCGCACCTGGTACCACGTTTGCGGCACGTCCTTCACGGGCGCCGAATCCTTCATCGCGAAGAACA GCATCGATTCGCCGGGCCGCGAGTAGCTGCGCAGGTAATCGATCGCGGGCATTTCCTGCAGCTTGCGGCC GATCCGGTCGGTGACCTGTTCCTGCACCTGGCGCGCGGTCGCGCCCGGCCAGAACGTGCGGATCACCATC ACACGGAACGTGAACGGCGGGTCTTCCGATTGCGCGAGGCGCGAGTAGGCGAGGATGCCCGCGATCGTCG CGAGCGCGATCAGGTAGACGACGAGCGCCTGATGGCGCAGCGCCCATGCGGACAGGTTGAAGCGTCCGCC TTCTTCTCGGCCGGCCGTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 3516264-3519434	Sequence	BBK_3067 - acrB/AcrD/AcrF family protein (Protein is coded in complementary strand; from 3519434 to 3516264)
ORF... 3519431-3520546	Sequence	BBK_3068 - efflux transporter, RND family, MFP subunit (Protein is coded in complementary strand; from 3520546 to 3519431)