Sequence retrieval

Genome: Burkholderia pseudomallei NCTC 13179
Start position: 241373
End position: 242098
Length: 726

DNA sequence
>NC_022659, from 241372 to 242097 (726 bp); Burkholderia pseudomallei NCTC 13179 TCAGCGGCCGGCGCGCGCGAGCTCATACACGCGTTGCGCGACGTCGGCCGCGACGGGCCGGCCGCCGCGC TCGGCACCGTACCGGTCGCGCCGGTCGCAGCGATCGGCCGGCCGCGCGAGGACCGCGGCGAGTTGCTCGG CGAGCGGGCGCGCCGGATCGAGCACCGCGTCCACCGCGCCCGTCTTCGCGAAGTTCTCGAGCCCCGGCGC GAACACCGCGGTCGATCGCGCCTTCTCGCGCAACACGTCGACGGGCAGCTTCGTCACGCGCGAGATCGCC TGCAGATCCATCACCTCCGGTTGCGCGCCCGGCAGCGCGACGAGCGTGCGGGTGGCGAGCGCCGTCGCGA TGAACGCGCCCGCCGCCGCGTGCCCGTACAGCAGCCCGATCGTCGGATGACCGAGCGCGTCCGCGTGCAG CAGGCACTTCGCGAGATGCGCGAGGCATTCGTTCAGGCCGAGCAGTTCGTCGCGCTTGCTCATCCGCTGG CTGTCGCCGTCGACGAGCACGAGAATCGGCGTCTCGCCGCCGCGCCGGATCGTCGCGAGCACGCGCTCGG CGAGCGCGAGCGCGCCGTCGACGCCGAGCGCCGCGCCATGCGCGACACCGATCACGTCGAGCGCGCGCCC CGCGAGCATGCCGTGCCCGGCAAGCAGGCCGGCGGCGTCGGCCGCGACGTCGTGCCCGGACGGAAACAGT GAAGCCAGCACGTCATCGAGCGTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 240460-241398	Sequence	BBK_187 mdcG malonate decarboxylase holo-[acyl-carrier-protein] synthase (Protein is coded in complementary strand; from 241398 to 240460)
ORF.. 241373-242098	Sequence	BBK_188 mdcE malonate decarboxylase, gamma subunit (Protein is coded in complementary strand; from 242098 to 241373)
ORF... 242085-243032	Sequence	BBK_189 mdcD malonate decarboxylase, beta subunit (Protein is coded in complementary strand; from 243032 to 242085)