Sequence retrieval

Genome: Burkholderia pseudomallei NCTC 13179
Start position: 240460
End position: 241398
Length: 939

DNA sequence
>NC_022659, from 240459 to 241397 (939 bp); Burkholderia pseudomallei NCTC 13179 TCATCGCCACGCCTCCAGAAACGTCCGCGGCGCGACCAGTTCGACGCCCGTCGCGGTTTTCATCGCGACT GCTCGCCCGCCCGCATGCAGTTCGCGCCAGTTCACGCCCGCGCCGTCCGCGCAAAGCAGCTCGCCGTCGA TGCGCATCGGCGCATCGGCATCGATCTCGGCGAGCGCCGCGAGAAACGGTGCGATCGCGTCGCGTCGCGA CGGCATCGACGAAGACCCCGGCCGCCGCGACAAACGCGATGCCGAAGCCTCCATTTGCGCGGTCATCGGC GCAGACGCCTCCGCTCCCGTCACCGCCGCCGACGACGACGCGCCCGCCAGCGCGGGCACCGGCCACGCGC GAGACGATTGCGGAATCGAAACCGACGGCGAGGACGGAGACACCGACACCGACGCAAACGAAGACGCAAA CGAAGACGCAAACGAAGACGAAAACCGAAACAAAGACGCGGACGAAGACGCCCCGCACGGCATCAGCAAG ACATCGAGATCCGACTCGTCGCTCAAATAGCGCAGCCCCGTGAGCGTCTGCCACGCGAGGCTGCCGAACA CGCGGCAGTCGACGCGATGCGCGTCGGCGAGCGCGCGCAGCCGCGCGAGCGTCGGCCGCCACGCGGGCGG CGCCTGCCGCCCGACGTCGGCGAGCGTCGGCAGCGCGCACGCCGAATCGATCTGCTCGCGCGCGGCGGCG AGCGCGACGCGCCGCTTCCCCGCCGACGGCGGCAGCGGCAGGCCAAGCGGCACGCCCGCGTCGCACGGCG AGCGCCGCCGCACGATCAGCGGCCAGCCGCGCGCCGCCCAGTCGCGCACGAACGGCAACGTGCCGATGCC GCCGTGCGCGGCGAGCAGCGCCGGCCACGCCTGCGCGCGCACGCGCACGAGCGTGTGCCGCACGAGCGAC GCATCAGCGGCCGGCGCGCGCGAGCTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 239096-240463	Sequence	BBK_186 mdcB triphosphoribosyl-dephospho-CoA synthase MdcB (Protein is coded in complementary strand; from 240463 to 239096)
ORF.. 240460-241398	Sequence	BBK_187 mdcG malonate decarboxylase holo-[acyl-carrier-protein] synthase (Protein is coded in complementary strand; from 241398 to 240460)
ORF... 241373-242098	Sequence	BBK_188 mdcE malonate decarboxylase, gamma subunit (Protein is coded in complementary strand; from 242098 to 241373)