Sequence retrieval

Genome: Actinoplanes friuliensis DSM 7358
Start position: 916767
End position: 918737
Length: 1971

DNA sequence
>NC_022657, from 916766 to 918736 (1971 bp); Actinoplanes friuliensis DSM 7358 TCAACCGTTCGGTCGGCCTCCACCGTGGTTCTGAGGAGGTCTCGCGAAACGCCTGTGACGCCCGCCCGCC TCTGGCACTCTGGCGTATGTTATGGCAAAAGCGACAAAAGGTTCCGCAGCTCCTCGGGCCGCCGCTGAGG ACCTGCCCACCCGGGACCAGACGACGGCGACCGTCCGGCGCAACCCGCCACCCTCCGCGGGCAAACCGCC AGTCTCCGCACGCCGTAAGTCGGCCTCCGCGCCTGACCCGGCGGTTACCGCGCTCAACCCGGCGGCCTCT GCTTTCGGCCAGGCGGCGTCCGTCCTTGATCCGGCGGCCCCCGTGCTGGGCCCGGCGGCGTCCGTCCTTG ATCCGGCGGCCTCTGCGCTCGGCCCGGCGGGCTCCGCGCGCAAGGCGGTAGCTGCGGGGCGCAACGCCCG TCGGCCGAGTGCCACCGCAGCCGGCCGTGCCCAGAATGCATCCCCACTCAACCCCACGGGGACACCCGCC ATGCCCGAATCACCTGACACCGCCGCACCCACGTCTGCTGCCTCGCCCGCGTCCACCGCCTTGCTTGCGT CAGGTGCTGCCGAGGCGAGTCCTGCTGCCCGCACGCGCCGGACCGCCGCCGCGGCAGGTGCCGAGCCGAC GCCCATCCGGCGCCGGAAGCCGGCCGCCGTCAAGGCCGCCGCAGCGCCCGCTGCCGAAGCTCCGAGTCCT GACGCTCTGGTTACCGAGGCCGCCAAGGCAGCGACAAAGGTCGCAGGCCGGCCCCGCTCGCCGAAGGAAC CGGAATCGCCGAAGCCGATCAGCGCGGCCCGGCCGGGCGCGAAGTCGGCCGGCCTGGCGTCCAACCCCGA AACGTCCGGCGTGGCCGAGGTTTCGGGACCAGCCCTGCCCGCCGCGAAGCCAGCACGCCAGGCCAGGCCT GCGGCCGCGAAATCTGCCGATCCGGTCGTGCTCGCGACCGCGAAGTCATCCGACCCAGCGAGGTCTGCGG TCGCGAAGTCCGCTGGCCCGGCCAGGTCTGCGACCGCGAAGTCGGCTGGTTCGGCCAGGTCTGCGGCGAC GAAGTCGGCTGGTCCGACGGGCTCGGCCGCGGTGTCTGACGTTGCGAAGTCGGCTGCAGCTTCGGGGATG ACGTCGATCGGCTCGGTGAGGGCTGCTCGTGTGACCCGGTCGAGTCTGCCCGCCGAATCAACCGGGACCG GCGCGGGGACCCCCGCACGGGCGGTTGCGGCCAGGTCTACGCCGAACAAGTCCGCCGTGACTGAGCGAAC GACGCCACCGGCGAAGTCGGCCCGGGCCGGTGCAGGGCCTGCCAAGTCAGCCGCCTCGGCTGGGCGGGCA GCCACCAAAGCTGCTGCGACCAAGAGCACGGGGTCGAGCAGGTCGGCGACTGCGGGAGCTACCGCCGGCA GGACGACGGCTGGCTCGGCGGCATCGTCGGCGGCGGCGAAGGCTGCCCCCGTGAAGACCGCTGCCGGAAC GCGGGCGCCTGCAAAGTCGGCTGCTGCCTCGGCTACCGGTGCGAAGCCGACGGCGGCGAAAGCCGCGACC ACGAAGGTGGCAGGGACAACGGCCGCTCCCGGCAAAGCTGCCGCAGCGAAGGCTGTATCGGCCAAGACCC CGGCCACCAAGGCCACACCTGCCAAGACCGCGACGGCCAGGACCGCGACCACAAAGACTGCGACGGCAAA GGGCACGACGGTAAGGGCCGCGACGGCAAAGGCCGCGCCTGCGAAGACGGCCGCGACGAAAACCGCCACG ACCGGAACCGCCACGACCGGAACCGCCACGACCGGAACCGCCACGACCGGAACCGCCACGACCGGAACCG CCACGACCGGAACCGCCGGGACCAAAACCGCCGGGACCAAAACCGCCGTCACCAAGGCGGCACCGACGAA GGCTGCGGCGGCAAAGGCAGCATCCGCAAAGACCACTGCGACGAAGGCAGCACCCGCGAAGGCAGCGCCC GCAAAGACCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 916767-918737	Sequence	AFR_04135 - hypothetical protein (Protein is coded in complementary strand; from 918737 to 916767)