Sequence retrieval

Genome: Actinoplanes friuliensis DSM 7358
Start position: 9063185
End position: 9063898
Length: 714

DNA sequence
>NC_022657, from 9063184 to 9063897 (714 bp); Actinoplanes friuliensis DSM 7358 TCATTCGGTCACCGTCACGCCGGTGTGGGTGCGCACGAGCCCGCCGAGCGTCGGTTTGTCGAGCGCCTTA CCGGTGTAGACGTGGCGTCCGCCGTCCGCACCGACAAACACGGTGACGGGCAGGTTGACCTTGCCGAGAC CGGTGAGCAGCTTCTTGTCCGGGTCGAACAACGTCGGGAACGACACGCCCTTGTCCGCGGCGAACGAAGC CCCGGCGGCCCGGGTATCGAACGTGTCGACGCCGACGACGTGCAACTGCCCAGCGGTGCGGTCGGCGAGC TCCTGCAGCAGCGGCAGTTCCTCCCGGCACGGCGCACAGGAGGAGGCCCACAGGTTGACCACGGCCGGCC CCCGCAACCCGGTCAGCTCAAAACTCTCCCCACCGGTAAAACACGGCAGCGCCACCGCCGGCAGATCCGC CGCCCCGGCTGAGGAAGAGGAACCGGCCACAGCCGAGCCCGACACCGCAGGCCCGGCCGAGTCAGAGGCT GAGCCCGGGAGGCCGGGCGCGCCCGGCGAGCCGGTCCCGGCCGGCGAGCCGGTCCCGGCCGGCGAGCCGG TCCCGGCCGAGGAGCCGGGCGTCGCCGAGCCTGCGCGGGCTGCGGCCGACGGGGTGGAGGTGAGCGCTGC GCAGTCGGCGAACGGTGACGCGGTGTTGTCGGTGGCTGACTGGGGTGGGGTTTTGGAGGCCGTGCAGGCG GCAAGGAGCAGCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 9062505-9063188	Sequence	AFR_41590 - hypothetical protein (Protein is coded in complementary strand; from 9063188 to 9062505)
ORF.. 9063185-9063898	Sequence	AFR_41595 - Thiol:disulfide interchange protein dsbE (Protein is coded in complementary strand; from 9063898 to 9063185)