Sequence retrieval

Genome: Actinoplanes friuliensis DSM 7358
Start position: 5412022
End position: 5415237
Length: 3216

DNA sequence
>NC_022657, from 5412021 to 5415236 (3216 bp); Actinoplanes friuliensis DSM 7358 GTGCTGCGCCCGGCCTCGCCGCTGACGTCCCTCCAGCAGTTCGAGTCCCCCGACGACGCCGCGGAGGCCC AGCTCATGGCGGACCAGCGCCCGGCCTCACCCGAACCCGCCCGCACCGGCGAGGACGCCCGTCCGGCCCC GGCCCGGGTACGCCCGGCGGCCCCCGAGGGCGTGGACCCGTCGCGCCCGGCGGCGACGGACATCCAGACC ACGTCCGGCGTGCCCAAGACCGCGATCCCGGCCGCCCCGGTCCCGACCGCCTCAGTGCAGGCCGCCCCGG CGCCGGTGGTTCCCGTCGTGCCGGGCCCGGCACAGACCGAGCCCGAGCCCGTATCCACCGACGAGCCGGC ACCATCCGCCGAAGCCGAGAGCGCCGACCACCCAGCAACGACGCCTGACGCCGACAAGACCACCGCCCAC CAGCCGGAGCCCGCCGAGGTTGGCGAGGCCGCTGAGACCGGCAAGGTTGCCGAGACCGCCGAGACCGCAG AGACCGCAGAGACCGCAGAGGTTGGCGAGACCGCCGCGACCGGCGTGGTTGATGGTGTTGGCGAGACCGC AGAGACCCCCAAGGTTGGCGAGGCCATCGAGGTTGGCGAGGCCATCGAGGTTGGCGAGGTTGGCGAGGCC GAGGACGTCGACTCCGACGGACAGTCATCAACGTCCGAAGCCGACGAGGCCGGCGAGGCCGAACCGGTGG CCGAGGTGGTTGAGACCGCCGACCCGGAACCCGCTGTCCAAGGCGTCGAGCAGCCGCCGGCGCCCGAGGT CGTCGAGGTGGAAGAAACGGAGGCCGCAGCCGAGACTGTCGAGCAGCCGCCGGCGGTTGTGGAGCAGGAG CCCGACGAGGCTGACGGAGACGCCAAAAAGGCCGACGCCGCGGAAGCTGTAGAGGCAGAGACGGCGAGCA CTGCCGGCGCGGACGTGACCGAAGCGCTCGCGGGTGAAACGGCAGCGGCAGATGCGGCCCCGGAAGACGA GTCCGAGGGTGTCACGCCCGAAGACGCGCCGTCCGGCGAAGCCGAGGGCGCCTCCGAGGTCATCGAGGCC GTCGAAACGGACAACGAGCAGGTCAGCACCACAGAGGACCAGGGCGATCCTCACCCTGAAGCTGAGGTTG AGGAAGGCAAAGCTGAAGCTCAGGTCGAGGGTGAGACGACGCCCGATATCGACCCGGAAGAGACCGCTGT CCTGATTCTAGCGGACCGGGACGCTGCCGACCTGGACGACACGACCGTCCTTCCGGCACCCGTCGAAAGG GCGATCGACGACACCGTCGTGATCCCGGCGTTCAGTGTCACGCCGCCGGTCGGGGTGGTCACGACCGTGA TCCCCGGCCGCCGGGCCGAGGCTGTCACCTGGGTTCCCCGGCCCGCCCGGGCCCGCCGCGCGCCTCTCGA TCTCGGGCATGCCCCCGCGGTGCTGACGATCTCGGCGCTGGGTGTGCTCATGGTTGCTCTCGCGTACGCC GGGGGGCGGGTCAGCACCGGCAATGCGCAGTTCGCGTACTGGGTGGGGCAGGTGGTGGTTTTCACCCCGG TGGTGGTGCGGCTGCTGAGCCGGCGCCTGGCCGGGGTGGCCGAGTCGTTCGTGCTGGTGATGGGACTGGC GCTGAACCAGTACCTGCTGAAGTGGATGTACAGCCCGGATCAGTTCCGGTTCCCGGACGAGCTGCAGCAC TGGCTCGCGACGACGATCATCGTTCAGTCGGGTGAGCTGTTCCAGCCCAATCCGGCGCTGCCTCCCGCGG AGCATTTTCCCGGGCTGGCCGAGCTCGGCGCCGCGGTGGCCCAGATGACCGGGCTGACGGTGACAGCCGC CGGGGTGATCGTCGCCGGGGTCGCCCACCTGGTTTTTGTCGGCGTGCTCTTCGCCGTGGTGCTGCGGGCG AGCAACTCCCCCGCCGTCGCCGGGGTGGCCTGCGCGACCTACGCGACCGCGCTGCACTATCTCTTCTTCA ACTCGATGTTCCTCTACCAGACCGCCGCGCTGCCGTTCTTCCTGCTGACGATCTGGGCCGTCCGGCGGTG GCGGGCCGAGGGCGGCCGGTCGTTCGCCGTCCTCGCGGTGCTGTCGATGACGCTGACCACGGTGAGCCAC CACGTGACGGCGCTGGCCCTGGTGGCGACGCTGACGCTGCTCGCGGCCGCCGAGCTGATCACCGACCGGC CGCGGCGCTGGTCCGCGCTGATCATGCCGGGTGCGGCCGCCGTGGTGGTCGCGTCGTGGATCCTGTTCGT CGCCACGGACGTTCTCGGGTACCTCGAGGACCCGGTGGACCAGGTTGTGCGTACCGTGTCGCAGCTTTTC TCGGGAGAGAGTGACGCGGCCTCGGAGAGCGCGCCGGTCTCCCTGGGGCAGTTGGCCGTTCAGGGTGCCG GGCTGCTGGGTCTGCTCGTGCTCGGCCTGGCGGTGACGCGGGACATGCTGCAGCGGCACGACCGGGACTG GTGGCGCTGGGCCGCGGTGGCCGGCAGCGCGATCTTCTTCGCGGGCAACGGCGTCCGGTTCCTCGGGCAG AACGGGCCGGAGATCGCCGGGCGGCTGTCCACCTTCACCTACGTGCCGATCTCCATCCTGGCGGCGATCG GCCTGGTCCGGGGTGTGCAGCTGATCCCGGCGCGGGACGCGGAGGGGCATCGCTGGCGGGCCGCCGCGCC CGCGATCGACGTGGTCGTGCCCGGCAGCTGGAACCTCTACAGCCGGGTCGCGGCCGGCGCGGCGATGATC ACGCTGCTGATGATCGGCGCGCGCGCCGGTGGGTGGCCGCCGATCGCCTCGCTCCTGCCGGGCCCGTACC TGGCCGGCGGTTACGAACGGTCCGTCGACGCGTACGGGCTGGAAGCGGCCGACTGGCAACGCCGCACGCT CGGGCCGGACCACCGGGTCGGCGGTGACGCGACCTCGGTCTCGCTCGCCTCGACCTACGGGCGGCAGGAC CCGGTGCGCGAGGTCGGGCCGTTGTTCTACGCCGACTCGTGGGGGCTGGAGCAGGACGCGCTGGTGCAGC GGCTGCACCTGCAGTACCTGGTCGTGGACCGGCGGCTCGGCACGCAGTTGCCGGAGAGCGGCTCCTACTT CGAGATGGATCCGCGGTCCGGGCAGATCACGTCGCCGCTCACCACCGGGCAGCTGGGGAAGTTCGACTCG CTGACGGAGGTGGACCGGCTCTACGACAACGGCATCGTCCGTGTCTACGGAACGGGGGTCCGGTGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 5412022-5415237	Sequence	AFR_25015 - putative transmembrane protein
ORF... 5415234-5416190	Sequence	AFR_25020 - hypothetical protein