Sequence retrieval

Genome: Actinoplanes friuliensis DSM 7358
Start position: 5216887
End position: 5218932
Length: 2046

DNA sequence
>NC_022657, from 5216886 to 5218931 (2046 bp); Actinoplanes friuliensis DSM 7358 TCACGGCACCATCCAGCTGAGCACCGGGGTGCCCTGCAACGTGACCAGCACACACATCACCAGCAGGAGC AGCAGGCTCCAGCCGACCACCTTGCGGAAGATGTCACCCTCACGTCCGGCCATGCCGACAGCCGACGCCG CGATCGCCAGGTTCTGCGGGCTGACCATCTTGCCCAGCACACCGCCCGACGAGTTCGCCGCGGCCAGCAG CACCGGATCGAGCCCGGCGCGGGCGGCCGTCTCGACCTGCAGCGCGCCGAACAGCGCGTTCGCCGAGGTG TCGGAGCCGGTCACGGCCACACCGATCCACCCCAGGATCGAGGACAGGAAAATGAACACCCCACCGGCCG CGGCCAGAAATTCACCCAGCGTGTTCGTCTGACCGGACTGGTTCAACACGTACGCGAGCGCGAGCACCGC CATCACCGTCACGATGGCGTGCCGCAGTTCGGCGTACGTGTGCAGGTAGGCCTTGAAAGCACGACCGGGA CCGATGCGCAGCACCAGGGCGGTCAGGATGCCCGCGAGGATCATCAGCGTGCCGGCCGCGGAGAGCCAGC CGAGGGTGAACGTGGTCGAGGCCAGCGGTTTGCCGTTGGTCCCGAGGACGTCCAGGCCCGGCCACGCGAA GGAGACCGACCACGGCGCCTTCGCGAGCGCCGCCTTCACCGCACCGATGTTGGCCACCGAGAAGATCACA ATGATGATCAGGTACGGCGCATACGCCCGCGCCACCTCAGCAGCACCGTCCCGCCGCGTCACCGTGGCAG CTCCCCCACCACCCACGGTGGCCTTCCGCGCCGAATCGACCTCCGTCGACCCAGCCGGACCTGATCCGGC CGCAGCGGGCGCAGCTGAGCCGGACGCGGCCCGACCGGAAGCAGGCGGACCGGACGCAGCTGGACCAGGC CCGGCTGAACCAGGCGAAATCCGGCTGAATCCAATCGGACCCGACTCGGCTGCATCAGCCGACCCAGCCG ATGCGACCGGACCTGACCCGGCCGGACCTGACCCGGCCGGACCTGACCCGGCCGGACCTGACCCGGCCGG ACCGGGCGCAGCCGAGCCGGACGCGGCCCGACTGGAAGCAGTCGGACCGGACCCAGCTGGACCAGGCCCG GCAGAACCAGACGCGGCCCGGCGCGACGCGGTCGGATCCGACTCGGCGGGTGGTTCGGCGTGGAGGTTCG GGGATTCGACGGGCTGCCAGATGCGCAGGAGCAGGACCACGGCGGCCGCCGCGAGCAGCGCGGCGATGAT GTCGGTGAGCGGCACCGAGATGTAGTTCGAGGCCACGAACTGGCCGAGGGCGAACACCACACCGGCCACC AGCGCCGCGGGCCAGGTCTGCCGGATGCCGCGGCGGCCGTCGATGACGGCGACCAGGACCAGCGGTACGA CGACGGCCAGCAGCGGTGTCTGCCGGCCGACCATCGCGCCGAGGGTGTCGACGTTGAGGCGCGGGTCGTC GGCGGCGCCCGAGGTGACCGTTCCGAGCGTGACGATCGGGGTGGCGAGCGCGCCGAACGCGACCGGGGCG GTGTTGGCGATGAGCGCGACAGCCGCGGCCCGGATCGGTGGGAAGCCCAGTGCCATCAGCATCACCACGG TGACGGCCACCGGGGTGCCGAAGCCGGCGAGGGCCTCGAGCAGGGCGCCGAAGCAGAACGCCACGATGAT CGCCTGGATGCGCATGTCGGGGCTGACGCGTTCCATCGAGCGGCGCAGCACGTCGAAGTGCCCGCTGGCG ACGGTGAGGTTGTAGACCCAGATGGCGTTGAGCACGATCCAGAGGATCGGGAAGAAACCGAATGCGGCTC CCTCACTCGCGGAGAGCAGCGCCTGTCCGACCGGCATGGAATAGACCGCGACGGCCACGATGAGAGCAAC TGCCAAAGCAATCAGCCCCGCGAGCCAGGCCTTCATGCGTAGTACTCCGAGCAGAATGAACAGAGTCAGC AGCGGCAGGACGGCGAACAGGGCCGAGAAGGCCACGGAATGGGCGACGGGGTCGGTCACGATCGTGAACT GATCCATGACAGCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 5215095-5216906	Sequence	AFR_24115 - histidine kinase
ORF.. 5216887-5218932	Sequence	AFR_24120 - L-lactate permease (Protein is coded in complementary strand; from 5218932 to 5216887)