Sequence retrieval

Genome: Actinoplanes friuliensis DSM 7358
Start position: 4554591
End position: 4555541
Length: 951

DNA sequence
>NC_022657, from 4554590 to 4555540 (951 bp); Actinoplanes friuliensis DSM 7358 TCACGGGGCCGCCAGGTCGGGGATGCGGGCGGTGACGCGGAATCCGGGCCCGGCCGGTTCGGCGTGGAGG GTGCCGCCGAGGGCGGAGACGCGTTCGTGCAGGCCGAGCAGGCCGTGGCCCGCGCCGGGTGCCGTCGGCC ATGGTCCCTGGGGGCCGGTGTCGCTGACGGCCGCGACGACGGACCCGTGGTCGCGGTAGACGTGCACCCG GACCGTGCTGGGGCCGACGTGGCGGGCGACGTTCGTGAGCGCCTCGCGGACGGTCCGGCACACGGCGACC TGGACGCCGGGGGAGACCTCGCCGACGGGCTCGAGTCGCAGGGAGGCACCGGTTCCGGCGACGATGGCGG GCAGCTGGTCGAGGCTGTCGACCGGCAGCCGTGGCGCGGTGTCGTCCGCCGAGCGCAGGACGGTCAGCAT GCGACGCAGCTCGGCGGTGGCTTCCCGGCTCGCGGCCTCGACGTCGGCGAGCACGTCAGCGAGCACGTCG GCGGGCGGTCCGGCGAGCACGTCGGCGGGCGGTTCGGCGGGCGGTTCGGCGGGCGGTTCGGCGGGCGGTC CGGCGGGCGGTCCGGCGGGCGGTCCGCCGGGCGGTTCCCGGGGATCGGCGGCTGTCAGGCGTGCCGCCGC CACGCGCACGGTGATGAGCCCGAGGCCGTGCGAGACGATGTCGTGGAGGTCCCGGGCGATCCGCAGACGT TCCGCCAGTACGGCCTCGGAGGCCGCCCAGCGGGTCAGCCGGGCCTCGTAGGCGGCGCGGTCCGCGCGGG TGCGGTGCACGGCCCAGGCGGCGACGCCGGCCGCGGTCACCGCGATCGCCGTCGGCAGCACCACCGTGAC CGGTCCCTCGCCGCCGGTCGCGACCAGCAGGAACACCAGCACGGCGAGCGCGGCGCCCGCGACGGGCCAC GCCGTCGATCGCCGGGCGGGCCGGTGCCGTTCGTGCTCCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 4553926-4554594	Sequence	AFR_20905 - two-component system response regulator (Protein is coded in complementary strand; from 4554594 to 4553926)
ORF.. 4554591-4555541	Sequence	AFR_20910 - histidine kinase (Protein is coded in complementary strand; from 4555541 to 4554591)