Sequence retrieval

Genome: Actinoplanes friuliensis DSM 7358
Start position: 4548337
End position: 4549023
Length: 687

DNA sequence
>NC_022657, from 4548336 to 4549022 (687 bp); Actinoplanes friuliensis DSM 7358 TCATCCCACCGGCTGAGCGGTGACGACGATGTTGTCGCGGTAGTGCCCGGTCTTCTTGTCGAACGGCCCG CCGCAGGTGATCAGGGTGAGCCGCGTGGCGCCGTCGCGGGCGAAATAACGGTCCAGGGGGATGCGCGTCT TCGCGTACCTTTCCCGGGCGATCACCTCGAATTCACGAATCACGCCGTCGGCATCCGCGAGGGATATCGG ATCACCGGGGGAGAGTTTGTCCAGGCCGAAGAATGCGCCTTTCCCCTGGGCCGCGCTGTCCACGTGCCCG GCGACGACGATCGAGCCGGCGTCGGCGGTCATGCCGGGCCCGAAGCGGTACCAGCCGACCCGGTCGACGC TCGGCGGCACGGCGAAGTCGCCCGTCTTCGCATCGACACCGACGGCGGCCACCGTCGCGTCCAGCTTCAT CGCGGGGATCCGCAGCCGGGCCGGGACGACCTCACCCGCGGCTTTCCGGGAAGGAGAGGGGCGCGCCGAG CTCGGCGAGGACGAGGACGAGGACGAGGACGAGGACGAGGGCGCCGGGGCCGAGGCCAGGGCCACGACCG CCGCGGTCCCGACGGACGGCTCGGGTCGCGTACGGACCCAGGCGATCCCGGCGCCGATCAGCAGCACCAG CCCGGCGATGACGAGGAGCAGGGCGCGCCCCCGGAAGGACGCGCCCGTCTTGTTCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 4548337-4549023	Sequence	AFR_20875 - putative sortase family protein (Protein is coded in complementary strand; from 4549023 to 4548337)