Sequence retrieval

Genome: Bacillus amyloliquefaciens CC178
Start position: 1146547
End position: 1147047
Length: 501

DNA sequence
>NC_022653, from 1146546 to 1147046 (501 bp); Bacillus amyloliquefaciens CC178 TTAGAGGACGAGGGTGATGACTAAGATGGCAATCAGTGTATTTGTTGCTGTTGAGATCAATGTTGCGATA TCGGCATCTACTGTTGTCACGTTTACGCCGCGGGAGTTTTCGATGAGCAGTTTTTGACGGTTGATTTGTT TGTTAGCTGTTAGCTGCAATAAGTCTTGTGCGACCAATTCAGCGATGACTCCGTCAGCGATTGAAGTGAG AGTGGCCGTTACGACTGCTGCATTAATCGCTGTCATGACTGAAGTGACTGCCTGAATATCTGTCGATGTC ACGTTAATATCACAAGAATCTCTGATAATAATCGCTTCTTCAGAAACTTGTTTGTTGAGATTCGCCTGTG CCAGGTCTTGGTCGATGAAGGCATTATCTTCGCCGAACCCGTGTCCGTTACAGCAAACACTGCATCTTCT TCCTTCTTTTTCTTCTTTTTCTTCTCGCGGATGTTCACATTCAGGATCAAGTGCAACCCAAGAGTATGGT CTGCTTTCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1146547-1147047	Sequence	U471_11740 - cotX (Protein is coded in complementary strand; from 1147047 to 1146547)