Sequence retrieval

Genome: Sorangium cellulosum So0157-2
Start position: 681541
End position: 682239
Length: 699

DNA sequence
>NC_021658, from 681540 to 682238 (699 bp); Sorangium cellulosum So0157-2 TCAATACTTGACGGTGCAGCCGAACGCCTCGGTCTCTTTCGTGGAGACCGGCTTTCCGGCGGTGAGCTCG GCGAGCGCCGCGTCGACGTAGTTGGTGACCTGCTCGTCCGGCTCGGGCTCACCGCCCTTCGTGCTGTCGA TGGCGCCCGCGTAGACGAGCGTGCCCTGCGGATCGATGACGTACATATGCGGTGTGCGCTGGGCGCCGTA CGCGCGCCCGACATCGCCCTTCTCGTCGATGAGGAGCGGGTAGCTCATCCCGAACTCCTGCTTTGCTTTC ACGTTCTCTTCCCGGGTGGTGCCCTGCTTGCCCGGAGCGCCCGAGTTGATGGCGAGCCACACGACGCCCT TCTGGGTGTGCTGCGCCGGCACGCCCTTCAGGCCGCCCTTCCTGTGGGCGAGCTGCACGAACGGGCAACC GGGGTTGAACCACTCGAGCACGACCGTCTTGCCCTTGTAGTCGGCCAGCTTGACGGGCTTCCCGTCGAGG TCCGGCAGCGTGAAATCGGGCGCGGGCTGGCCGATCTTCGCCTGGGTCGCCGGCGCCTGGGCCGCCTTCG CCTCGGCGGGCTTCGCCTCGGCGGGCTTGGCGGGCTGCGCCGCCGCGGCGCCGTTCGCCGCCGGCGCGCT GGGCTCGCCGGGCTGGGTGCACGCCGCGAGCGCCGCGGCGAGGAAGGTCAGGGGAATGAGCTTGTGCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 681541-682239	Sequence	SCE1572_02485 - hypothetical protein (Protein is coded in complementary strand; from 682239 to 681541)