Sequence retrieval

Genome: Propionibacterium acnes HL096PA1
Start position: 142018
End position: 144486
Length: 2469

DNA sequence
>NC_021085, from 142017 to 144485 (2469 bp); Propionibacterium acnes HL096PA1 GTGCTGCTGGGCGCGGCACCGGGCGTCGGCAAGACCTACGCCATGCTTGACGAGGGCAAGCACCTGCGCG CCGGGGGGTCTGATGTCGTTATCGCCGTTGTTGAGACTCACGGCCGGGCAGCTACTGCGGCAATGACCGA GGGGATGGAGGTCATCCCGCGGCACTACGTCGAGTGCCGTGGCATTGAGCTGCCGGAGATGGATCTTGAC GGGGTGCTGGCCCAGCACCCTGACGTCGCCCTCGTCGACGAACTTGCCCACACCAACGCCCCCGGGTCCC GTAATGAGAAGCGTTGGCAGGACGTTGAGGAGCTTCTGGAGGCCGGGATTGACGTCATCTCGACGGTCAA CATCCAGCACATCGAGTCCCTCAACGACGTCGTTGAGCAAATCACTGGGGTACCACAGCGCGAGACTGTC CCGGACACCGTGCTGCGCAGGGCCAGCCAGGTGGAGGTCGTCGACCTCGCCCCGCAGGCCCTGCGCGACC GGTTGTCGAGCGGCAAGGTGTATCCGGCCGAGCGGATCGACGCGGCCTTGTCAAACTACTTCCGGCTCGG CAATCTCACCGCCCTGCGTGAGTTGGCCTTGTTGTGGGTGGCCGACGAGGTCGATCACGCGCTGGTCGAC TACCGTAGCGAGCACGGCATCGACTCCCGTTGGGAAGCCCGTGAACGGGTAGTCGTCGCCCTGACAGGGG GTCGGGAGGGCGAAACCCTGCTGCGACGCGGTGCCCGGATCGCGGTTCGAGCCGGTGGCGGAGAGTTGCT GGCGGTGCACGTCACCACCGAGGACGGGCTGCGCTCCCCGCATCCGGGGGAACTAGCCCGGCAACGAGGG TTGGTGGACAAGCTAGGAGGGTCCTTCCACCAGCTCGTTGGCGATGACATCCCCGAGACTCTCATCGAGT TCGCGAAGTCGGTCAACGCCACCCAGCTCGTCATCGGGGCTAGTCGGCGTGGCAAACTCTCGCGGCTGTT GACCGGACACGACGTCGAGGCAGACATCATCCGGGCTTCCGGTGCTATCGACGTTCACATCGTCAGTCAT GACGCCCAGGCGAAGGCGCTACGTTTGCCGCGGATGGGTGGGTCGTTGTCGACGCGGCGTCGGATCGCTG GTTTCGCTGTGCTGGCGGTGCTCGGGCCACTCTTGACGTGGCTGTTGGTGTCGTCGCGTAGCCCGGAGGC GCTGACGACCGATGTGCTGGCTTACGAGTTGCTGGCCGTCGTGGTGGCCTTGGTAGGTGGGGCATTTTCG TCGCTCATTGCGGCTCTGGCCTCGGGTTTTGCGCTGGACTATTTTTTCACCAAACCTTTCTACACCGTCA CCGTCAACGAACCGTTACACCTGGTGGCACTGTTGCTGTACCTGCTCACCGCGGCCCTGGTGAGTGTTGT TGTCGATCGCGCTGCCCGTAAGACCCGGGTGGCTCGACGCGCCGCCGCTGAGTCGGGACTGTTGCAGTCG GTGGCCGGTTTGGTGCTGCGCGGCGAGGACGCCGTTGAGTCGTTGTTGTCCAGGGCGACTGAGGCTTTCG GGCTATCTGGGGCGCGATTGATGACCGACGGAAAGGTTGTGGCAAATTGGGGTACAACGAGTGATCAAGT CACGGAGCATCGCATCGACGACCATACCGTGGTGGAATTCCACGGCCCGGAGCCGGACGCCTCGGAGCGT CGGCTGATGTCGGTCATCGCCGCCCAGTTGGGGACAGCCTTGGAGCAGCATCAGCTGGAGGAGACGGCGA AGGCGGTGGAGCCGTTAGCGGCCACCGACCGGGTGCGCACGGCGTTGTTGTCAGCGGTCGGTCACGACCT GCGTCGCCCCCTGGCGGCTGCCACCGCGGCTGTGTCTGGGCTGCGCAGCGAGTGGTCGAAGCTTTCTGAC CAGGATCGTGCCGATTTGTTGGAAACCGCTGATGAGGCGTTGTCGCAGTTGGCGAATCTGGTCACCGACC TTCTCGACGTCTCCCGGTTGCAGTCGGGGGTGCTGGGGGTCTCGGTGATGCCGGTAGACCCGGCTGAGGT CATCATGCCGGCGTTGGACGAGTTGTCCCTGGGGCCGGCCGACGTCGAGATTGACCTGGGTTCTGACGTT CCCGAGATGGTCGCCGACCCAGCCTTGCTGCAGCGAGTTGTCGTCAACCTGTTGTCGAATGCGCTGCGTT ACGAGCCGGCGGGCAGCCGGGTGCGGTTGGCGGAGAGTAGCTTCGCCAACCAGGTGGAGATTCGAGTCAT CGATCACGGCCCGGGGATTCCAGAGGACCGTCGCGACGATGTCATGGTGCCTTTCCAGCGGCTGGGTGAT ACGGATTCCAGCGTAGGGCTGGGGCTTGGCCTGGCGCTGTCGAAGGGGTTCGTCGAGGGAATGGACGGCA CCCTGGCCTTGGAGGACACTCCCGGCGGGGGACTGACGATGGTGATCAGCTTGCCCCGGGTAGGGGTTGA GGAGGCAAGTGTTCAATGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 142018-144486	Sequence	PAGK_0118 - two-component sensor, KdpD
ORF... 144483-145166	Sequence	PAGK_0119 - two-component response regulator, KdpE