Sequence retrieval

Genome: Bacillus subtilis subsp. subtilis 6051-HGW
Start position: 3574344
End position: 3575765
Length: 1422

DNA sequence
>NC_020507, from 3574343 to 3575764 (1422 bp); Bacillus subtilis subsp. subtilis 6051-HGW TTATTGAACAACACGTCTTACAACACCTTTAAATGCTGCTTTCCAGTAAGGATTGCTCATAGAGTCAACA GATACGCCATGAGATGTATTGTCGTTTAGGAAAGTACCGTTTCCTAAGTAGATTCCTACGTGTCCATTTG TTTTGTAAGTGTCAAAGAACACAAGGTCTCCGCGTTTCATTTCAGACGCGCTGACAGCTTGTCCTCTGCC AACTAACGTATCAGTTGTTGTTCCGCCTACAGGTCCAAGGTTAACACCTGCAGAAGCGTATGCCCAGCGT ACGAATGATGAGCAGTCAAAAATACGGTTGTTGATATCAGACTGAGTGCGTCCGCCGCCAAACTTGTACG GAGATTGTCCTACAATGCTTGAACCAACGCTGATCGCGCCTTCAATTCCGCCAGAGTTGCTGATAACAGT GCCGCCTGAATTGCTGTTTGAGCCGCTGCTCTTCTTAGAAGATGAGCCGTTTGACGAACTGTTTGATGAA CCGTTAGAAGAATTGTCTGAAGAATTGTCTGAAGAATCATCAGATGAGCTTGCTTTTGATGATTCAGTTG TCGCCGTTTGACTGTCATCTGTTTGTGTTTCATCAGATGCTTTTTGTGCTTCTTCCTGCTTTTTGATCAA AGCAGCCGCTGCTTCTTGTTCTTTTTTGATGCGTGCTTGTTCAGCTTCAGTATTTGCTTTTTGATTTGCA AGTTCAGACGCCTCTGATTTTAATTCAGAAATCGCTTTAGCCGTTTTCTTTTGGCTTGCTTTTGCTTCGT CGAAAAGCTTGTCTTTTTCATTAAGCTGTTTATCAAGGTCTTTTTGCATTGTTTCTAATTTAGCCAAGGC AGCTTGAACTTCTTTCAGCTTGTCATTCAAATCCGCTTCAGAATCTTCGAGCTTCGCTTTATCCTGCTCT TGCTGTTTGATTAAATCTTTGTCTGCGTCCACAATAGATGAAACCGCAGTCGCACGAGAGATAAAGTCAC CAAAGCTTGTTGATCCTAAAAGAACATCTATGTATCCTTGAGATCCGCCGCTTTCCTGTAAAGAACGAAC GCGTTTTTTCAGGATTTCATTGCGTTTTTCGATGCGTGCTTCAGTTTCTTTAATCTCTTTTTTCAGTTTT TTGATTTCTTCTTTTGTTTTATCGTTTTCTTCTTTTTTATCTTCAATCTTGTTGCTTGTATCAAGCGCCT TATCGTTAATGTCTTTCAGTTCTTTTTCAATCTTTGATTGATTTTCCTGAAGCTCGGTTAATTCTTTTTC CTTCGCTTCAATGCTGGAAGCAACCTCAGATTGCTTGCTTTCGATTTTTTGTTTCTTTTCATCTAATGTT TCCGCCGATGCAGTTTTACTTGTAAATGGGATCAAAAAACTGCTTGTCCCGATGACGGAAGCCAAACCAA GTGTAATTAAACTCTTTCTCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 3574344-3575765	Sequence	BSU6051_34800 cwlO secreted cell wall DL-endopeptidase CwlO (Protein is coded in complementary strand; from 3575765 to 3574344)