Sequence retrieval

Genome: Bacillus subtilis subsp. subtilis 6051-HGW
Start position: 1250653
End position: 1251171
Length: 519

DNA sequence
>NC_020507, from 1250652 to 1251170 (519 bp); Bacillus subtilis subsp. subtilis 6051-HGW CTAGAGGACAAGAGTGATAACTAGGATGGCTACGAGTGTATTTGTTGCTGTAGAAATAAGGGTTGCGATA TCGGCATCTACTGTTGTGACGTTTACGCCGCGGGAACATTCGATGAGAAGTTTTTGGCGGTTTACTTGTT TGTTAGCTGTAAGCTGTAACAAATCTTGTGCGACTAATTCGGCAATTACGCCGTCTGCAATTGATGTCAG AGTTGCCGTTACGACAGCGGCATTAAGTGCTGTTACAACTGATGTTACGGCTTGAACGTCTGTAGATGTA ACATTGATGTCACAAGAATCTCTAATAATAATCGTTTCATCTGAAACTTGTTTGTTGAGATTTGCTTGAG CTAGATCTTGGTCGATGAAGGCGTTGTCGTTGCCAAAAAAACCATTGCCATTACAGCAAACGTCGCAATG ACATTTTCTTTCGTGTTTTTCTTTATCTTTCTCTTTGTCATCTAACGGATGGTCACAGTCAGGATCAAGT GCAACCCAAGAATATGGTCTGCTTTCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1250653-1251171	Sequence	BSU6051_11760 cotX spore coat protein CotX (Protein is coded in complementary strand; from 1251171 to 1250653)