Sequence retrieval

Genome: Pseudomonas poae RE*1-1-14
Start position: 5225566
End position: 5226117
Length: 552

DNA sequence
>NC_020209, from 5225565 to 5226116 (552 bp); Pseudomonas poae RE*1-1-14 ATGACACGAATCAATCTTTTGCCCTGGCGCCAGGCGCTGGCGCAGCGGCGGCGCAAATACTTCCTGGTAT TTTTGCTGGCGTTCGTCTGTGTGGCGCTGGCGGCGGTGTGGCTGGCGGATCAGGTCATCGACCAGGCCAT CGACCGCCAGGTAACGCGCAACAGCCATGTGGGCAAGGACGTCGCCGCCCAGGATGCTCGGATCAATACC ATCGACGACCTGCAGGAGCAAAGCCAGCAACTGGCCGAGCGCATGAAGGTCGTGCAGGACCTGCACGAAG CCCGCTCTGCCGGCGCGCAGTTGCTGGACCAGTTGGCCCGCGCCGTGCCCGAGGGCGTGCATCTGCACGA GGTGGTTGCCCGTGGTGACAGCGTCAGCATCAGCGGCAGCGCTGAATCCAGCCAGGATATCGCTCAACTG ATGCGCAGCCTGGAAGCCTCCCAGGGCGCCCACGCGACGCGGCTGCAACATGTGCGGGCGGAGGGGGCAG ATGGCCGCAACGAATTTCAATTGATGGTGCGCCAGGGGGAATCCACCGAGGCCCAGCCATGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 5224913-5225569	Sequence	H045_22915 - type IV pilus assembly protein PilM
ORF.. 5225566-5226117	Sequence	H045_22920 - type IV pilus biogenesis protein PilN
ORF... 5226114-5227085	Sequence	H045_22925 - type IV pilus biogenesis protein PilOP