Sequence retrieval

Genome: Pseudomonas poae RE*1-1-14
Start position: 5140147
End position: 5141745
Length: 1599

DNA sequence
>NC_020209, from 5140146 to 5141744 (1599 bp); Pseudomonas poae RE*1-1-14 TCAGCGCGGCTTTAGCACGTCCACACGCAAGGTGGAGGGCTGGTCCGCCTGGTCGTAGGCGTAGGTGCAG ATCTTGTAGGGCATGCCCTGGACGCTGATCAATTGCGCGTATTTTTCCGGCGGCGTGGCCGGGTCATCGT TGAGTGGGTGCACCAGGGCGATGACCTCTTCGTCCTCGTCTTCGTCTTCCTTCACCGGCGTCGGCGTCGG CGGTATGGGCAGATAGTCCACCGGACGCAGGCAATCGGTGGTGGCGGCCGGACCGTACTTGTAGGTGTAA CGCTGCATGAAGGGCGGTGCCAGGTGCCAGACCAGCGTATAGGCCGCAAGGCCCTGTTTATCCAGGCTGA TGCTCAGGTTCGGTGTACGCGCCGAGCCCGGTTCGGCCAGTTCGACGGCCAGGCTCAGCGCATTTTTCAT CATGCCCGACGTAACGCGCTGCTCGGCGGACTCCACGCCGACGATACACAGCATGTGCATGCCGGTTTGC GGGCCGATGGGGTCATTGACGTATGCATGGGCAGCCTTGATCGGCACGCTGTAGAGCGCCGGGTTTTCGC AGTCGATGGCTCGGCGCGTGGTTTTGTAGCGATAGAACGGCGTGTTGATGCTGAAGGGCAGGTTCCAGCG CGGCACGATGTCCTTGCCCCTGCCGTCGGCTTTCTTGAGGAAGTACTGCTGGATGGGTGTCGGTTTTTTG AACGTGATGGAGGTGGTCGGGTACAGCTCGCAGTGCTCGCCATCCGTCTGCAGCACACCGCCGACAAAAC ACTGGTTGAGGAACGTTATCGGGCTGCCGTAGTTGGTCTCGGTCTGCCAGACGGGTATGCCGTTATTGAC CTCGCATGGCGCGTCGCGCTGGCATTTGCGCTCGGCGGGCTGCCCGCGGGTGCTGGTACTGAGGATGCCG AAGAGCCGGTTGCTGGCGCGGTCGAGCAGCGGCCCGCCAAAGCCGCCGAGGGCGACGCCCTGACATTGGT TCTTCATCGTGACTCGCCACACGAACGGCTGTTCCACCAGTTCCCGCGAGGTCTGTTGAGTGCAGGCCGC CAGGTGCAGGGTGTCCCATTCGGGAGCGGTGAGGGCGAGGATATCCACTCCGTCAGCGGGTATTTCCTCG GCCAGTTTCAGCGGCGTGATGCCCGCCGTGATCAGGCTTGCCAGGGTCGCCTGCAATTCAATCACGGCCA GGTCGACCCCGTGGCTGCTGCCCCATCTCAGGGTTTTCAGCGGGTAGGTCTTGAGGGCCTCAAGGGTGTC GTCGAAATAGTTGAAGCTGATGCTGCCCTTGATCGACAAATCCCGTTGCACCGCGCCGTTGAGGCGATGC AGGCAATGACTGCTGGTGACCACATAAGCGGGGCCGGACTTGCCGCTCTCATCACGGGTGTCCAGCAGGC TGGCGGTGCACAACGTCTGACTTTCATTGCGGATGCGGCCGACGCCGTTCCAATGGTCATGTTGATGCCT GGCGTTACGCAGAATCTGTGCCGGGCCGCTATTGGCCAGGCCTTCGCCGAAATCCTTGTTGGCGGCGTGC AGTGCGCCGCACAGGCTGGCCAGCAGCAGCCCAGAAGCAACAACGTTTTTAAGGTTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 5140147-5141745	Sequence	H045_22540 - putative lipoprotein (Protein is coded in complementary strand; from 5141745 to 5140147)