Sequence retrieval

Genome: Pseudomonas poae RE*1-1-14
Start position: 4160332
End position: 4161552
Length: 1221

DNA sequence
>NC_020209, from 4160331 to 4161551 (1221 bp); Pseudomonas poae RE*1-1-14 TCAGCTCAGGCGCAGGCGGCTGGCGCCCACCAGCCGGTTGTAGATCAGGTACAGCAACACGGTGGCCAGC AGCAGCAGGCCGCCCAATGCCGTGGCCATGCCCCAGTTGATGCTGGTGTTGGTGTAGAACGCCACGAAGT AGCTGACCATCTGGTCGTTCGGGCTGCCCAGCAGGGCCGGGGTGATGTAGTAACCAATCGCCAGGATGAA CACCAGCAGGCAGCCGGCGCCGACACCGGCGTAGGTCTGCGGGAAGTACACCCGCCAGAAACTCGCGAAC GGATGGCAGCCCAGGGAAATCGCCGCACGCATGTAGGTGGGCGAAATCCCCTTCATCACGCTGTAGATCG GCAGAATCATGAACGGCAGCAGGATGTGCACCATGGAGATGTAGACCCCGGTGCGGTTGAACACCAGTTC CAGCGGGCTGTCGATCAGGCCCAGGCCCATCAGCGCGCTGTTGATCAGGCCGCCGGACTGCAGCAACACG ATCCACGCCGCCACCCGCACCAGAATCGACGTCCAGAACGGCAGCAGCACCAGAATCATCAGCAGGTTGC TTTTGCGCGCCGGCAGGTTCGCCAGCAGGTAGGCCAGCGGATAGGCCAGCAGCAGGCAGATCACGGTGAT CACCAGGCCCATCCAGAAGGTACGGGCGAAGATGTCCAGGTAGATCGCCTGGTCAGGCGTGGCCGGAGCG ATCTCGCCGAGGTCGTCGATACGGTGATCGACGGCCGCCAGCAGGTAGTACGGCGTCACGCTGCTGGTAT TGCGCCGGATCGCCTGCCAGTAAGCCGGGTCGCCCCAGCGTTCGTCGAGGTTTTCCAGTGCCTGTTTATA AGAGCCGGGCGTTTCGGCAAACGGCAACGCCCGCGCGGTTTTCGTCAGCAGGCTGCGATAGCCGGCCAGT TCCATGTTCAAGCGTTTGGACAGATCGCCCAGGGTCTGGTTTTTACGCGCCTCGCCCAGGTCTTCGCTGA GCGCCTGGTACACCGGTTCGCCCGGCAGGCCGCGTCCGTCCCACGCCGTCACGGCCACCACGGTACGCGG CAAACCGCCCACCACTTCCGGGTTGCCGACACTCTTGAACAACAGCGCGACGATCGGCACCAGAAACACC AGCACCAGGAACAGCACCAGCGGCGCAATCAAGGCCTGGGCCTTCCAGCGGTTGAGCCGCTCGGCGCGCG CCAGGCGCTGCTTGAGGGTGGGGATGGCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 4160332-4161552	Sequence	H045_18215 - putative ABC transporter membrane protein (Protein is coded in complementary strand; from 4161552 to 4160332)