Sequence retrieval

Genome: Pseudomonas poae RE*1-1-14
Start position: 1187472
End position: 1188236
Length: 765

DNA sequence
>NC_020209, from 1187471 to 1188235 (765 bp); Pseudomonas poae RE*1-1-14 TCATTGCACGCTGTAGTTGAAGGTGGCGGTAAAGCGCTGCTCATTGCGCCCCCCGAAAGCTTGCTCGGGG AACGGTTTGACTTGCTTGATGCGCCGCAGGCTGTTGGTGGCGGCGCGGTTGAGCAACATGCTCGACGCTT GGGTTTGCAGGCCTGAATCCAGCACCCGGCCCTGGCGGTCGACCAGCAACCACACCACCACCTCACCGCT GGGACGTTCGAGTGACGCCTGGCGCCCTGTGGGGTACTGCTTGTAGGTGTCCAGATCGTTGCGTAAGCCC TTGAGATAACCGCCTTCCAGGGCCTGGCCGTCGACCTTTGGCGGTGCCGGTGCCGGTGCCGGTGCCGGTG CCGGTGCCGGTGGCGCGGGTGTCGGGGCGACTGCGGCAGGTGTCGGTTTGATCACGGCCGGTGCAGCGGG CTTGGCGGGTGCAGGGGACGGCGCCTGAGTCGGCTTGGCGGCCACGACCGGCTTGGGCACAGGCTTGGGT TTGGGCTCGGGTTTTGGCGCGGGCTTAGGGGGAGGTGGCGGTGGCGCAGGTTCTGCCTCTTCATCCTCGA TCACGGGCGGCGGCGGCGGTGGCTCTACCACAGGTTCCGGGATCACTTGCGGCTCCGGCTCGACCAACGC CAGCTCGACCGCCGACTCGTCGTACACCGGTTCGACTTTCAAGGTCTGAGACTGGATGCCCATCGCAATC AACACCAGGGCGATCACGGCCGGGACGCTTCCCAGCAGCTGCCGCACACGAAACAGGCCGTACAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1187472-1188236	Sequence	H045_05515 - TonB domain-containing protein (Protein is coded in complementary strand; from 1188236 to 1187472)