Sequence retrieval

Genome: Enterococcus faecium NRRL B-2354
Start position: 1117335
End position: 1118171
Length: 837

DNA sequence
>NC_020207, from 1117334 to 1118170 (837 bp); Enterococcus faecium NRRL B-2354 ATGAACGTCAGCTATCTAGGGCCAGAAAGTTCATTCACTTATCAGGCGGCTTGTCAGCTATTTCCTACAG AACAATTGACTGCGTATGCTTCCATCCCTGCTTGTTTGCGCGCTTTGTTTCGCAAACAAGTCGATCTGGC TGTCGTTCCTGTAGAGAATTCATTGGAAGGATCTGTTCATCACACAATCGATCTTCTTTCCAAACATCCA GAAGTCGAAGTTAAAAGTGAAATCGTTCTTCCTATCAAGCAGCAGCTTTTAGGCAATCCAGCAACAAAGA TTACAAAAATCCTTTCACATCCACAAGCACTAGCACAATCACAACAGTTTTTAGAAACGCATTATCCTAA TGTTCCTTTAGTAGCAACAGAATCAACAACTGCTGCAGCAATGTATGTAGCAGAACATCCGAAAGAAGAC GCAGCAGCGATTGCTTCATTAGAAACAGCACAGCATGTTGGATTGGAGATCTTAGCTGAGAATATCCAAG ATAATGAGTTGAATCAGACACGTTTTTGGATCATAGGTGATCGAAAGATGACCAGCCAGCAGCCAGCTCC AGTAAAAATGAGTGTCATCTTGACACTACCTGCTAATCGGCCAGGGATGCTTCATAAAATGCTGGCAGCT TTCGGATGGCGGGAGATCAACTTAAGTAAGATTGAATCCCGACCATTGAAAACCAGTTTGGGAGAATATT TTTTCGTGATCGATTTGTTGTTGGACCGCCCAATGACATTAGTAGAGAATGCGCTCCAAGAAATCAAGAT GCTCGGAGGAGAAAGTCAAATATTGGGCTGTTATCCAGTGCTGACAGTAGAAGAGACGCGTAGATGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1116829-1117338	Sequence	M7W_1278 - Shikimate kinase I
ORF.. 1117335-1118171	Sequence	M7W_1279 - Prephenate dehydratase