Sequence retrieval

Genome: Escherichia coli APEC O78
Start position: 774647
End position: 776257
Length: 1611

DNA sequence
>NC_020163, from 774646 to 776256 (1611 bp); Escherichia coli APEC O78 TCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGTAGC AGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCA GGGTACGGAAATCATCGTTACCGAACAACGCCACTACGCCAAAATCACCAATCGACAGCACGCAGGCAAA AGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCA ATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCA GCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAAT GGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCG CTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCA TCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCA CAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACGCCATCGACGATCACCGCCAGTAACGGT GGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCG GGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGTAC CAGCCCAAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTC AGCGCCTGATAAATAGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCGCCCAGTGATAGCACGGTGGCGA AGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTC GACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATG TTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCAGATTAAAAAATACGTGCGCCAGCAAAATAC CTTGCAGGCCGTAGGGCGAAAAGGTCCACTTCAGACCGAGCGATTGGCAGAGCGATGCCAGCCAGCCCTG ACGACCATAGACGCTAAGAATGCCGAAAACAGCAACTAGCACCGGGAGGATCAAGGTCATTGCGCACAGA CGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGGGCGCGGGCGAGGAATATCGCGGGTACGA CAGAGAGTAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGGTAGCTGTCCTG CCAGACTGCCACCCAGTTACCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCGAGCGCAACCGCT ACCACCAGCGTGGCGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCA T

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 773965-774663	Sequence	APECO78_03780 thiQ thiamine transporter ATP-binding subunit (Protein is coded in complementary strand; from 774663 to 773965)
ORF.. 774647-776257	Sequence	APECO78_03785 thiP thiamine transporter membrane protein (Protein is coded in complementary strand; from 776257 to 774647)
ORF... 776233-777216	Sequence	APECO78_03790 tbpA thiamine transporter substrate binding subunit (Protein is coded in complementary strand; from 777216 to 776233)