Sequence retrieval

Genome: Escherichia coli APEC O78
Start position: 4751314
End position: 4752519
Length: 1206

DNA sequence
>NC_020163, from 4751313 to 4752518 (1206 bp); Escherichia coli APEC O78 TTATTCTCCTTGCGGCGCAGCTGCCGAAGTATCAGCTTGCGGTGCAGGTGCAGGTGCAGGTGCAGGTGCA GGTGCAGGTTTAGCTTCCGTTGTCCCCGCTGCCGGTTGTGCCAGCAGGTTACGCACGCGAGTCTGCATCA GTTTTTCCAGCATCGCCTGGCTTTGCAGGGTTTCCGGAAGATCCATCGAGATATTTTGCTGGCTTAACTG GTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGTACCCAGGTGGAG ACGTTCTCCAGCGCCTGGCGATAAGTCTCTTGCTGGTGACGCGGTACAGCTTGTGCTGCGACCAGCAGGC GAGAGCGAATATTTTCGCGCAGATAGATATCCTGATTTGGCGCTAACAGCGGTACGGCGGTGTCATCACG ACGGCGAATCGTAATGAAGTTGTCCATAAAATTCTGCCAGCTTTTTTGCAGATTGATACGCCATTCGCTG ATGGAACTGGAAAGCTCTTCACCGTCGGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCA GGTTATCTACCTGATTTGAAAGCTGATTGAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACCGC AGAAAGGCTGGCGATATCATCGGTAATTGCCCGGCGAACGGTAATCAGACTCGGGTCATTCATATCCGCC AGGCTGGCATCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCTTGATCGCTCCACAGCTTCCGTC CGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGC GACCTTTTGTTGGACTTCATCCAACTGTTTTGCCAGCGTTTCTTGCTGACGATTCGCCTGCTTAAGCTGT GCAGCTTGTTGCTTAATAATGCCTTCCAGCTCGGCTTTTTGGCTCTCCTGGGCTTTTTGCAATGCCGTCA GTTGGTTAGCCAGGGCATCGCTGGTGGCGGTCTGATTGACGGCCTGTTGTTTACCCCAGCCATACAAACC GACGCCCGCCGCCAGAGCAATAGCGATAGCCACCGCGCTGAGAATCAATGCGGTATTGTTCTTACTCTTT TTTTCTGTTGCGACAGGTTGTGACGTGGTGTCCACGGCCTCCCTGGTCTCTTCAACCACGGCGGAGGTTT TTTCTTGTTCCGTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 4751314-4752519	Sequence	APECO78_22845 - uroporphyrinogen III C-methyltransferase (Protein is coded in complementary strand; from 4752519 to 4751314)