Sequence retrieval

Genome: Escherichia coli APEC O78
Start position: 3734277
End position: 3734873
Length: 597

DNA sequence
>NC_020163, from 3734276 to 3734872 (597 bp); Escherichia coli APEC O78 TCATACTGCCGAGACAATACAGTATGAGAAGGAATAGCATCTCTCCCCAATCTGATAATAAAGATATGAT ATCTCCATCAAGAGTTTTAAAAAGCAGTGATTTATGATTACTTCAAAATACTTTGATCGCCAATGCAAAA AAAAAAAAAAAAAAAAAAAATAAAAAAATAAAATAATATAAAGAATTGCTTTAACAAATTCTTTTACGGT TTTTAAGCCAAATATACGCTTTAAGCCATTTACTGGATTCAATGAATCAAACTTAAATTTAACAGCTTCA GTTGCTAATTGCATTTTACTTTGAAACCAGGAGACAAGTATTGTTGTGAACATGCAAACCAGCAAAAAAG GAATGATCGTTTTCAACCAACCAACCCCCATAGCTTTAAAATAGTCCCAAATATCTGGTTTAGCGTTATG ATCAAAGATGTATTCAAGAATCGACATAATGTGATGCACATCAAAAACATATCCGAGATATAAAGTACCC ACAAGCATTATTACAGAGATGGTTAAGTCTCTACTTTTTAGAATTTGCCCTTTTTTAGAAGCATCCTGGA GTTTCTTTTGTGTTGGCTTTTCAGTTTTATTGGCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 3734113-3734280	Sequence	APECO78_17955 - type III secretion system protein (Protein is coded in complementary strand; from 3734280 to 3734113)
ORF.. 3734277-3734873	Sequence	APECO78_17960 - type III secretion system protein SpaS (Protein is coded in complementary strand; from 3734873 to 3734277)