DNA sequence >NC_019970, from 1639736 to 1639840 (105 bp); Thermoanaerobacterium thermosaccharolyticum M0795 ACAAGTTTTGTTTTATTCTTTACAAGTTTTGTTTTATTCTTTACAAGTTTTGTTTTATTCTTTACAAGTT TTGTTTTATTCTTTACAAGTTTTGTTTTATTCTTT Translate to protein Restriction digest BLAST Design primers with primer3