Sequence retrieval

Genome: Brachyspira pilosicoli P43/6/78
Start position: 919311
End position: 920693
Length: 1383

DNA sequence
>NC_019908, from 919310 to 920692 (1383 bp); Brachyspira pilosicoli P43/6/78 TCAAGAGCCTGATAATTTTTTTGCATAATCGTTACTAACCTTTACTATATTACCAGCCCCCAAAGTAATT ATTATATCTCCGTCTTTTTTTATTTTTTCTAATTCTGGTAATATATCTTCTATATTTTTTATATATTTTA TATTATTATTTTTTACTATTTTGCATATAGTCTCACCGCTAATTCCAGCAATAGGACTTTCTCCTGCCGC ATATATATCGGTAATTATCAAATTATCAGCATCTAAAAAAGCATTTTCAAAATCATTTAAAAGCAGCTGA GTTCTGCTGTATCTATGAGGCTGAAATATAGCTATAATACGTCTGTCTTTATAAGCATTTCTAAGCGAAG ACAATGTAGCTTTTATTTCTGTAGGGTGATGTCCATAATCATCAAATAATGTTATCTCTTTATCATATAA TTTATTTAATCTTCTGCCTACACCTTCAAAAGTTTTAAGTGCTTTTTTTATAGTATCAGTATCTATATCT AAATGATATGCTACAGCGATTGATGCTAAAGAATTAAGTACATTATGCTCCCCTATTAAAGGAAGTTCAA ACTCGCCTAAAAACTTATCTTTATAATAAGCTTTAAAATATGTTTTAAACTCTTCTGTTTTTATAGAGCT TTTATCAACATAAAAATCATATTCATTAGAAAATCCATAAGAATAATATTTTTTACTTACACTTTTAGCC AAATCTTTTACAACATCATTTTCAAAACAAAGAAAAGAACAGCCATAAAAAGGAACTTTATTAATAAAAT CCAAAAAAGCAAGTCTTAATTTTTCAACATTGCCGTAATAATCTAAATGGTCATTATCTATATTTGTAAC TACGCTAAATACAGGTGAAAGTCTTAAAAAGCTTCCGTCACTCTCATCTGCCTCGCAAACCATATATTCA CTATTCAAATTACAATAAGCATTGCTCTTAAGATTAAAATGATTTCCGCCTATTATACATATAGGGTTTA ATTCAGCTTCTATCATTATTTGACTTATTAAAGAAGTGGTAGTAGTTTTACCATGAGAGCCTGATATTGC TATGCCTTGTTTTAAACGCATAATTTCAGCAAGCATTTCCCCTCTTGGTATTACAATAATATTTTTCTTT TTAGCTGATATTATCTCTTCATTAGTTGGGCTTATTGCAGAAGAACTAACAACTGCTGTAACATCATCTT CTACATTGCTTTCTTTATGACCATAATAAACTTTTATGCCGCAATCTTCAAGCGACTGAGTTTTTGGAGT TTTAGCTAAATCGCTTCCTGTAATAGTAAATTCATCTATAGAATTAAGAACCAATGCTATAGCACTCATT CCTATTCCGCCTATTCCTATAAAATGTATCTTCTCTTTCTTTTTTGTAAACAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 918407-919336	Sequence	BPP43_04380 - UDP-N-acetylmuramate dehydrogenase (Protein is coded in complementary strand; from 919336 to 918407)
ORF.. 919311-920693	Sequence	BPP43_04385 - UDP-N-acetylmuramate alanine ligase (Protein is coded in complementary strand; from 920693 to 919311)