Sequence retrieval

Genome: Brachyspira pilosicoli P43/6/78
Start position: 155028
End position: 155753
Length: 726

DNA sequence
>NC_019908, from 155027 to 155752 (726 bp); Brachyspira pilosicoli P43/6/78 TTACAAATCATTTTGAAGCCTCATTATAATTTCAAATATAATATTAAATATAATTGCAATCAAACAATAT GCAAACAAACTAACAGTGTTTAAATATAAATGCGATTCATATAGTTTTGTGCCGAGCCCTAATGTTGGCA GAGTTATAATTTCTGAAGCTAATACGCTTTTCCAAGTCATGCCCATTGATTGCGATATTGAAGTGAGTAT ATATGGTTTTATTGATGGTATATACAAAGTTATTAATTGATTTTTAAAAGACACATTATAAGAGTTTGAC ATTTCTATGAGATTTTTATCAATGTTTATAATGCCATTTACTACAGCATCATATATTATTGGAAATATTA CAAACAAAGATACAAATATAGGCACTAAATTATTATTAAACCATAATACTGCTATTAAAATAAGAGGTAT TGTTGGAATTGTTCTAATAAAGTTTATGATTGGCATTAAAATATATCTAACACTTTTAAATATACCAGAA ATTATTCCTAATGTTATAGCAAGCAAAAATGAAAGAACAAAAGTAACTATCACCCTTATAAAAGTATATA ATAAATTAGAGTAAAAATCTTTTTGAGAAATTATATTTATTAAATCTAAAAAAATCTTATCTATACTTGG GAAAATAATTTCAGAATTAATTTTTAGAGAAATAAAACTCCATATAGCTATGAATAATATTATGCCTACA AAAAAATATATAATCTTTTTTTTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 154340-155038	Sequence	BPP43_00740 - nitrate transport ATP-binding protein, NrtD (Protein is coded in complementary strand; from 155038 to 154340)
ORF.. 155028-155753	Sequence	BPP43_00745 - binding-protein-dependent transport systems inner membrane component (Protein is coded in complementary strand; from 155753 to 155028)