Sequence retrieval

Genome: Alcanivorax dieselolei B5
Start position: 4549742
End position: 4551694
Length: 1953

DNA sequence
>NC_018691, from 4549741 to 4551693 (1953 bp); Alcanivorax dieselolei B5 ATGACGACGTCTTTTTCCCTGCCTCGCCGGCGCTTCGTGCAGGGCCTGGCCCTGGGCGGCACCGCTCTGG GTCTGGGGCTCACCCCGACGCAATTGTTCGCCCGCCAGGGCCAGACCGGCGCGCCGGAGCTGCGTGGCAA CCAATTCCAACTGAACATTGGCGCCGCCGATGTCAATCTGACCGGCCGCAACCGGCCCGCCACCCTGGTC AACGGCAGCCTGCCGGCGCCGACCTTGCGCTGGCGCGAAGGCGAGACGGTGACGTTGAGAGTGACCAATC ACCTGCCAGAGACCACCTCGATCCATTGGCACGGCCTGCTGCTGCCGTTCCAGATGGACGGCGTACCGGG GTTGTCCTTCGACGGTATCGCCCCCGGCGAAACCTTTACCTACCAGTTTCCGCTGCGGCAAAGCGGCACT TACTGGTATCACAGTCACTCCGGTTTTCAGGAGCAGAGCGGCCTGTACGGCGGCCTGATTATTGATCCGG CGGAAGCGGACCCGCATACGGTGGATCGCGATTACGCGGTGGTGCTTTCCGACTGGAGCGACCAGGATCC CAAGCGCATCTACGCCACGCTGAAAAAACTCAGCCACTACTACAATTTCAGCGAACGGACGCTGTTCGAC ACGCTGAGGGATTTCCGTGAGAAAGGCGTGGTGCGGACGCTCAAGGATCGCCACATGTGGAACCGGATGC GCATGAGCGAGACCGATCTGGCGGATGTGACCGGTTACACCTACAGCTTCCTGGTCAACGGCCATACCCC GGCCGCCAACTGGCAAGCCCGCTTCGAGCCCGGCGAGCGCATCAAGCTGCGCTTCATCAACGCCAGCGCC ATGACCATCTTCGACCTGCGTATTCCCGGCCTGGATATGACCGTGGTAGCCATGGACGGCCAGCCAGTGA AACCGGTGCGCTTCCACGAGTGCCGCCTGGGTGTGGCGGAAACCCTGGACGTGATCGTCGAGCCGAAGGA TCACCGCGCCTATACCCTGTTCGCGCAAACCATCGACCGGTCCGGCTACGCCCGTGGCACCCTGACGCCG GATCCCTCGCTCAGTGCCGAAGTGCCGGAGCTGGACCCGGTGCCGCTGCTCGGGCACCGGGAAATGGGCA TGGGCGCCATGGATCACGGAGCCATGGGACACGACAGTGGCGCCATGAAGACCATGGACCACTCTCGCAT GAATCATGGCGGCATGGACCATGGGGATATGAACCATGGCGATATGTCCGGCGGCATGAATCATGGAAGC ATGAACCATCAGGGGATGAGCCACGGCACGCAGGACGGGATGGACCACTCGGCCATGGGGCACACCAACC ACGACACCAACGCCGCCGTGCCCTTCGCGGTCTCGGCGGACAACCCGGATCTGCCGGCCCGACAAAGCGC CACGGAACACGCCGACAGCGAATACGGTCCTGGCGTGGACATGCGTGCCATGTCTCCGGTGCCGATGCTC GACGATCCCGGCATCGGGCTACGCGACCGGGACTGGAAAGTGCTCACCTACGCCGACCTGGAATACGCCC ATGGTCTGCCCGACAGCACGCAGCCGGAGCGGGAAATCGTGTTGCACCTCACCGGCAACATGGAACGCTA CATGTGGAGTTTCAACGGCATCCCGTTCGCCGACGCCGAGCCGCTCGAGCTGTATCACAACGAACGAGTG CGGCTGACCCTGGTGAACGACACCATGATGCACCACCCGATCCACCTGCACGGTCTGTGGAGTGATCTGG AACTGGGCGATGGGCGTCTGCTGCGCAAACACACCCTGTTGGTGAAACCCGGAGAAAAACTCAGCTATCT GGTTCGTGCCGATGCCCTGGGACGGTGGGCCTACCATTGCCACCTGCTCTATCACATGGAAGCGGGCATG TTCCGGGAAGTGCGCGTGGTGCCACGCCATCAACACCACACCGACGAGGAGAGCCGCTCATGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 4549742-4551694	Sequence	B5T_04104 pcoA twin-arginine translocation pathway signal:copper-resistance protein CopA
ORF... 4551691-4552419	Sequence	B5T_04105 pcoB Copper resistance protein B